Mitochondrial targeting sequence of magnetoreceptor MagR:More than just targeting  

作　　者：Yanqi Zhang Peng Zhang Junjun Wang Jing Zhang Tianyang Tong Xiujuan Zhou Yajie Zhou Mengke Wei Chuanlin Feng Jinqian Li Xin Zhang Can Xie Tiantian Cai 

机构地区：[1]High Magnetic Field Laboratory,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei,Anhui 230031,China [2]University of Science and Technology of China,Hefei,Anhui 230026,China [3]Department of Anatomy,School of Basic Medicine,Anhui Medical University,Hefei,Anhui 230032,China [4]Institutes of Physical Science and Information Technology,Anhui University,Hefei,Anhui 230039,China [5]Key Laboratory of Tropical Translational Medicine of Ministry of Education,NHC Key Laboratory of Tropical Disease Control,School of Tropical Medicine and The Second Affiliated Hospital,Hainan Medical University,Haikou,Hainan 571199,China [6]International Magnetobiology Frontier Research Center,Science Island,Hefei,Anhui 230031,China [7]Institute of Quantum Sensing,Zhejiang University,Hangzhou,Zhejiang 310027,China

出　　处：《Zoological Research》2024年第3期468-477,共10页动物学研究（英文）

基　　金：supported by the National Natural Science Foundation of China(31640001 and T2350005 to C.X.,U21A20148 to X.Z.and C.X.);Ministry of Science and Technology of China(2021ZD0140300 to C.X.);Natural Science Foundation of Hainan Province(No.822RC703 for J.L.);Foundation of Hainan Educational Committee(No.Hnky2022-27 for J.L.);Presidential Foundation of Hefei Institutes of Physical Science,Chinese Academy of Sciences(Y96XC11131,E26CCG27,and E26CCD15 to C.X.,E36CWGBR24B and E36CZG14132 to T.C.)。

摘　　要：Iron-sulfur clusters(ISC)are essential cofactors for proteins involved in various biological processes,such as electron transport,biosynthetic reactions,DNA repair,and gene expression regulation.ISC assembly protein IscA1(or MagR)is found within the mitochondria of most eukaryotes.Magnetoreceptor(MagR)is a highly conserved A-type iron and iron-sulfur cluster-binding protein,characterized by two distinct types of iron-sulfur clusters,[2Fe-2S]and[3Fe-4S],each conferring unique magnetic properties.MagR forms a rod-like polymer structure in complex with photoreceptive cryptochrome(Cry)and serves as a putative magnetoreceptor for retrieving geomagnetic information in animal navigation.Although the N-terminal sequences of MagR vary among species,their specific function remains unknown.In the present study,we found that the N-terminal sequences of pigeon MagR,previously thought to serve as a mitochondrial targeting signal(MTS),were not cleaved following mitochondrial entry but instead modulated the efficiency with which iron-sulfur clusters and irons are bound.Moreover,the N-terminal region of MagR was required for the formation of a stable MagR/Cry complex.Thus,the N-terminal sequences in pigeon MagR fulfil more important functional roles than just mitochondrial targeting.These results further extend our understanding of the function of MagR and provide new insights into the origin of magnetoreception from an evolutionary perspective.

关 键 词：Magnetoreceptor(MagR) N-terminal sequence Mitochondrial targeting signal Iron-sulfur cluster 

分 类 号：Q75[生物学—分子生物学]