Spatially resolved transcriptomic profiling of placental development in dairy cow  

作　　者：Guang-Hui Tan Shi-Jie Liu Ming-Le Dou De-Feng Zhao Ao Zhang Heng-Kuan Li Fu-Nong Luo Tao Shi Hao-Ping Wang Jing-Yuan Lei Yong Zhang Yu Jiang Yi Zheng Fei Wang 

机构地区：[1]Key Laboratory of Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China [2]Key Laboratory of Livestock Biology,Northwest A&F University,Yangling,Shaanxi 712100,China [3]Key Laboratory of Animal Biotechnology of the Ministry of Agriculture,Northwest A&F University,Yangling,Shaanxi 712100,China [4]College of Information Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China

出　　处：《Zoological Research》2024年第3期586-600,共15页动物学研究（英文）

基　　金：supported by the National Key R&D Program of China(2022YFF1000100);Technology Application and Development Program for Rapid Propagation of Cow Breeding(20211117000005);Basic Science(Agricultural Biology)Research Center of Shaanxi(K3030922016);Ningxia Hui Autonomous Region Key R&D Projects(2021BEF01001);Natural Science Basic Research Program of Shaanxi(2022JQ-171)。

摘　　要：The placenta plays a crucial role in successful mammalian reproduction.Ruminant animals possess a semi-invasive placenta characterized by a highly vascularized structure formed by maternal endometrial caruncles and fetal placental cotyledons,essential for full-term fetal development.The cow placenta harbors at least two trophoblast cell populations:uninucleate(UNC)and binucleate(BNC)cells.However,the limited capacity to elucidate the transcriptomic dynamics of the placental natural environment has resulted in a poor understanding of both the molecular and cellular interactions between trophoblast cells and niches,and the molecular mechanisms governing trophoblast differentiation and functionalization.To fill this knowledge gap,we employed Stereo-seq to map spatial gene expression patterns at near single-cell resolution in the cow placenta at 90 and 130 days of gestation,attaining high-resolution,spatially resolved gene expression profiles.Based on clustering and cell marker gene expression analyses,key transcription factors,including YBX1 and NPAS2,were shown to regulate the heterogeneity of trophoblast cell subpopulations.Cell communication and trajectory analysis provided a framework for understanding cell-cell interactions and the differentiation of trophoblasts into BNCs in the placental microenvironment.Differential analysis of cell trajectories identified a set of genes involved in regulation of trophoblast differentiation.Additionally,spatial modules and co-variant genes that help shape specific tissue structures were identified.Together,these findings provide foundational insights into important biological pathways critical to the placental development and function in cows.

关 键 词：Spatial transcriptomics Dairy cow PLACENTA GESTATION 

分 类 号：S823[农业科学—畜牧学]