Functional dissection of parabrachial substrates in processing nociceptive information  

作　　者：Jin Ke Wei-Cheng Lu Hai-Yang Jing Shen Qian Sun-Wook Moon Guang-Fu Cui Wei-Xin Qian Xiao-Jing Che Qian Zhang Shi-Shi Lai Ling Zhang Ying-Jie Zhu Jing-Dun Xie Tian-Wen Huang 

机构地区：[1]Shenzhen Key Laboratory of Drug Addiction,Shenzhen-Hong Kong Institute of Brain Science,Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong 518055,China [2]CAS Key Laboratory of Brain Connectome and Manipulation,Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong 518055,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Department of Anesthesiology,State Key Laboratory of Oncology in South China,Guangdong Provincial Clinical Research Center for Cancer,Sun Yat-sen University Cancer Center,Guangzhou,Guangdong 510060,China [5]University of Science and Technology of China,Hefei,Anhui 230027,China [6]Department of Anesthesiology,Shenzhen University General Hospital and Shenzhen University Academy of Clinical Medical Sciences,Shenzhen University,Shenzhen,Guangdong 518055,China [7]School of Medicine,Yunnan University,Kunming,Yunnan 650091,China

出　　处：《Zoological Research》2024年第3期633-647,共15页动物学研究（英文）

基　　金：supported by the Shenzhen Key Laboratory of Drug Addiction (ZDSYS20190902093601675);CAS Key Laboratory of Brain Connectome and Manipulation (2019DP173024);National Natural Science Foundation of China (82274358);Shenzhen-Hong Kong Institute of Brain Science;Guangdong Basic and Applied Basic Research Foundation (2023B1515040009)

摘　　要：Painful stimuli elicit first-line reflexive defensive reactions and,in many cases,also evoke second-line recuperative behaviors,the latter of which reflects the sensing of tissue damage and the alleviation of suffering.The lateral parabrachial nucleus(lPBN),composed of external-(elPBN),dorsal-(dlPBN),and central/superior-subnuclei(jointly referred to as slPBN),receives sensory inputs from spinal projection neurons and plays important roles in processing affective information from external threats and body integrity disruption.However,the organizational rules of lPBN neurons that provoke diverse behaviors in response to different painful stimuli from cutaneous and deep tissues remain unclear.In this study,we used region-specific neuronal depletion or silencing approaches combined with a battery of behavioral assays to show that slPBN neurons expressing substance P receptor(NK1R)(lPBNNK1R)are crucial for driving pain-associated self-care behaviors evoked by sustained noxious thermal and mechanical stimuli applied to skin or bone/muscle,while elPBN neurons are dispensable for driving such reactions.Notably,lPBNNK1R neurons are specifically required for forming sustained somatic pain-induced negative teaching signals and aversive memory but are not necessary for fear-learning or escape behaviors elicited by external threats.Lastly,both lPBNNK1R and elPBN neurons contribute to chemical irritant-induced nocifensive reactions.Our results reveal the functional organization of parabrachial substrates that drive distinct behavioral outcomes in response to sustained pain versus external danger under physiological conditions.

关 键 词：Lateral parabrachial nucleus Substance P receptor Pain affect Defensive reaction SOMATOSENSORY 

分 类 号：R338[医药卫生—人体生理学]