中朝边境地区野鼠中蜱媒病原体感染调查  

Tick-borne pathogens infection of rodents at the border of China and the Democratic People’s Republic of Korea

作　　者：王卓 吴琼 黄潇杨 黄冠鹏 马悠馨 毛恩荣 冷国双 赵洪明 李兵 吴益民 WANG Zhuo;WU Qiong;HUANG Xiao-yang;HUANG Guan-peng;MA You-xin;MAO En-rong;LENG Guo-shuang;ZHAO Hong-min;LI Bing;WU Yi-min(Biosafety Section of Center for Disease Prevention and Control in Northern Theater Command,Shenyang 110034,China;Changchun Chengtou Ecological Environment Technology Co.,Ltd,Changchun 130033,China)

机构地区：[1]北部战区疾病预防控制中心生物安全科,沈阳110034 [2]长春城投生态环境科技有限公司,长春130033

出　　处：《中国人兽共患病学报》2024年第4期323-327,共5页Chinese Journal of Zoonoses

基　　金：国家科技重大专项(No.2016ZX10004203G001G009)。

摘　　要：目的了解中朝边境地区野鼠中蜱媒病原体感染情况。方法采用PCR方法对吉林省集安、辽宁省宽甸林区采集的野鼠标本进行斑点热立克次体(SFGR)ompA基因、查菲埃立克体(Ec)与粒细胞无形体(Ap)16S rRNA、新埃立克体(CNm)groEL基因、巴尔通体(Ba)rpoB基因、土拉弗菌(Ft)fopA基因检测。结果PCR检测鼠脾标本共132份,SFGR、Ec、Ap、CNm、Ba和Ft的阳性率分别为9.85%、12.88%、5.30%、3.79%、51.52%和6.06%,不同病原体感染率差异有统计学意义(χ^(2)=149.236,P=0.000),以Ba感染率最高,表明该地区野鼠Ba感染高度流行。不同鼠种对SFGR、Ec、Ap、CNm和Ft的感染率差异无统计学意义;但对Ba感染率差异有统计学意义(χ^(2)=13.36,P=0.010),以黑线姬鼠感染率最高。132份野鼠标本中,2种病原体的复合感染率为15.9%(21/132),以巴尔通体的复合感染形式居多(15/132);检测到3种病原体的复合感染2份。结论中朝边境地区野鼠中6种蜱媒病原体感染普遍,存在2~3种病原体的复合感染,说明存在多种蜱媒病原体感染风险,可能存在多种蜱媒传染病的混合自然疫源地。To investigate the existence of tick-borne pathogens infection of rodents at the border of China and the Democratic People’s Republic of Korea(DPRK).PCR was used to detect the spotted fever group rickettsiae(SFGR)ompA gene,Ehrlichia chaffeensis(Ec)and Anaplasma phagocytophilum(Ap)16S rRNA,Candidatus Neoehrlichia mikurensis(CNm)groEL gene,Bartonella(Ba)rpoB gene,and Francisella tularensis(Ft)fopA gene in rodents samples collected from Ji'an of Jilin province and Kuandian of Liaoning Province.The positivity rates of 132 wild rats spleen samples,SFGR,Ec,Ap,CNm,Ba,and Ft were 9.85%,12.88%,5.30%,3.79%,51.52%,and 6.06%,respectively,with statistical differences in infection rates(χ^(2)=149.236,P=0.000).The infection rate of Ba was the highest in wild rats in this area.There was no significant difference in the infection rate of SFGR,Ec,Ap,CNm,and Ft among different rats species,but there were significant differences in the infection rate of Ba(χ^(2)=13.36,P=0.010).The infection rate of Apodemus agrarius was the highest.Among 132 wild rats specimens,the coinfection rate of the two pathogens was 15.9%(21/132),with Ba as the main species(15/132),and two cases of coinfection with three pathogens were detected.The infection of six tick-borne pathogens is common in wild rats at the China/DPRK border.Co-infection of two or three pathogens indicates a risk of multiple tick-borne pathogens and mixed natural foci of multiple tick-borne infectious diseases.

关 键 词：野鼠 蜱媒病原体 复合感染 聚合酶链反应 

分 类 号：R376[医药卫生—病原生物学]