新疆乌鲁木齐市莱姆病血清流行病学调查  

Seroepidemiological survey of Lyme disease in Urumqi City,Xinjiang,China

作　　者：杨丽[1,2] 张紫怡[1] 邵彤彤 李新婷 郑嵘炅 王世一[3] 王远志 郝琴[4] 鲁晓擘[1] YANG Li;ZHANG Zi-yi;SHAO Tong-tong;LI Xin-ting;ZHENG Rong-jiong;WANG Shi-yi;WANG Yuan-zhi;HAO Qin;LU Xiao-bo(Department of Infection and Liver Disease Center,the First Affliated Hospital of Xinjiang Medical University,Urumqi 830000,China;Department of Respiratory and Critical Care Medical Center,the Eighth Affliated Hospital of Xinjiang Medical University,Urumqi 830000,China;School of Medicine,Shihezi University,Shihezi 832000,China;State Key Laboratory of Infectious Disease Prevention and Control,Department of Spirochetosis Control National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]新疆医科大学第一附属医院感染与肝病中心,乌鲁木齐830000 [2]新疆医科大学第八附属医院呼吸与危重症医学科,乌鲁木齐830000 [3]石河子大学医学院,石河子832000 [4]中国疾病预防控制中心传染病预防控制所螺旋体病控制室,传染病预防控制国家重点实验室,北京102206

出　　处：《中国人兽共患病学报》2024年第4期334-339,共6页Chinese Journal of Zoonoses

基　　金：自治区重点研发任务专项(No.2022B03014-2);国家自然科学基金(No.82060115);新疆维吾尔自治区自然科学基金重点项目(No.2022D01D19);省部共建中亚高发病成因与防治国家重点实验室项目(No.SKL-HIDCA-2022-GR1)联合资助。

摘　　要：目的了解新疆乌鲁木齐市莱姆病在人群中的血清流行病学状况。方法选取2022-2023年乌鲁木齐市周边地区3个乡镇的体检人群血清795人份,其中芦草沟镇383人份、水西沟镇145人份和托里乡267人份,检测人群血清抗体IgG和IgM,采用酶联免疫吸附试验(ELISA)进行初筛,初筛阳性者行蛋白免疫印迹法(Western blot,WB)进行确认,收集WB阳性者临床资料,综合分析进行病例诊断。结果ELISA共计检出阳性110人份,阳性率13.84%(110/795),男性、女性阳性率分别为10.69%(37/346)、16.26%(73/449),女性血清抗体阳性率高于男性(χ^(2)=5.076,P=0.024)。不同年龄分层中的血清抗体阳性率差异无统计学意义(χ^(2)=2.569,P=0.766)。水西沟镇、芦草沟镇和托里乡的血清抗体阳性率分别为17.98%(48/267)、14.48%(21/145)、10.70%(41/383),其中托里乡的血清抗体阳性率高于芦草沟镇(χ^(2)=7.041,P=0.008)。将ELISA初筛阳性的110人的血清进一步行WB确认实验(分别检测IgG、IgM蛋白条带),共检出阳性82人,阳性率10.31%(82/795)。依据莱姆病诊断标准共确诊莱姆病患者20例,粗患病率为2.52%。结论乌鲁木齐市人群中存在莱姆病的感染和流行,感染率高于全国平均水平,建议进一步加强莱姆病的调查研究和防治。The prevalence of Lyme disease in endogenous populations in Urumqi,Xinjiang was investigated.In total,795 serum samples were collected from residents of three townships in the surrounding area of Urumqi City from 2022 to 2023,which included 383 from Lucaogou Town,145 from Shuixigou Town,and 267 from Tori Township.Serum levels of IgG and IgM antibodies were screened with an enzyme linked immunosorbent assay(ELISA)and confirmed by western blot(WB)analysis.Clinical data of WB-positive individuals were collected and comprehensive analysis was conducted for case diagnosis.The chi square test was used for statistical analysis of the results and the P<0.05 was considered statistically significant.In total,110(13.84%)of 795 samples were positive.The positivity rates was higher in females than males[16.26%(73/449)vs.10.69%(37/346),χ^(2)=5.076,P=0.024],while there was no significant difference among age groups(χ^(2)=2.569,P=0.766).The positivity rates for serum antibodies in Shuixigou Town,Lucaogou Town,and Tuoli Township were 17.98%(48/267),14.48%(21/145),and 10.70%(41/383),respectively,with a significantly higher rate in Tuoli Township than Lucaogou Town(χ^(2)=7.041,P=0.008).Of 110 individuals who were initially positive for IgG and IgM antibodies with the ELISA,82(10.31%)were confirmed positive by WB analysis.In total,20(2.52%)patients were diagnosed with Lyme disease based on clinical manifestations.Lyme disease is epidemic among the population in Urumqi,as the infection rate is higher than the national average.Hence,continued surveillance is recommended for prevention of Lyme disease.

关 键 词：莱姆病 血清流行病学 乌鲁木齐市 

分 类 号：R377[医药卫生—病原生物学] R514[医药卫生—基础医学]