α-酮戊二酸对湖羊精子质量与血浆生化指标的影响  

Effects ofα-Ketoglutarate on Sperm Quality and Plasma Biochemical Indices of Hu Sheep

作　　者：王赫川 崔卫国 张涵 李天峰 尹国安[1] 郭庆 李井春[1] WANG Hechuan;CUI Weiguo;ZHANG Han;LI Tianfeng;YIN Guoan;GUO Qing;LI Jingchun(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Heilongjiang Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《饲料工业》2024年第11期55-61,共7页Feed Industry

基　　金：黑龙江省自然科学基金项目[LH2022C068];伊春市重点科技计划项目[G2022-4];黑龙江八一农垦大学横向研究课题[2022050]。

摘　　要：试验旨在研究东北地区秋季饲喂α-酮戊二酸对湖羊精子质量与血浆生化指标的影响,选择体况良好且体重相近的湖羊32只,随机平均分为4组,分别按只补饲0、5、10、20 g/dα-酮戊二酸。试验于8—10月在黑龙江伊春市开展,平均气温10.3℃。预试期7 d,正试期56 d。分别于试验第28、42、56天采集湖羊颈静脉血,评估血浆睾酮、褪黑素、白细胞介素(2、4、6、10)、促肾上腺皮质激素、肿瘤坏死因子-α、丙二醛水平、总抗氧化能力及抗氧化酶活性。采集湖羊精液检测精子活率、精子质膜完整率及精子畸形率。结果表明:α-酮戊二酸对湖羊精子畸形率、血浆睾酮、白细胞介素(2、6、10)水平无显著影响(P>0.05)。每只湖羊补饲20 g/dα-酮戊二酸显著提高了精子活率、精子质膜完整率、血浆褪黑素水平、总抗氧化能力及超氧化物歧化酶、谷胱甘肽过氧化物酶及过氧化氢酶活性(P<0.05),同时显著降低了血浆丙二醛、促肾上腺皮质激素、白细胞介素-4及肿瘤坏死因子-α水平(P<0.05)。说明东北地区秋季每只湖羊补饲20 g/dα-酮戊二酸能够改善精子质量、血浆生殖激素水平、抗氧化能力与免疫指标水平。This experiment was aimed to investigate the effects of feedingα-ketoglutarate on sperm quality and plasma biochemical indices of Hu sheep in autumn in Northeastern China.A total of 32 Hu sheep with well body condition and similar body weight were randomly divided into four groups,each Hu sheep was fed with 0,5,10 mg/d and 20 mg/dα-ketoglutarate separately.The experiment was conducted in Yichun,Heilongjiang from August to October,with an average temperature of 10.3℃.The transition period was seven days,and the experimental period was 56 days.The jugular vein blood of Hu sheep was collected on the 28th,42th,and 56th experiment days respectively to assessed plasma testosterone,melatonin,interleukin(2,4,6 and 10),adrenocorticotropic hormone,tumor necrosis factor-α,malondialdehyde content,total antioxidant capacity,and antioxidant enzymes activity.Hu sheep semen was collected to test sperm viability,sperm plasma membrane integrity,and sperm abnormality rate.The results showed thatα-ketoglutarate had no significant effects on sperm abnormality rate,plasma testosterone,interleukin(2,6 and 10)content in Hu sheep(P>0.05).Supplementary feeding for each Hu sheep 20 g/d ofα-ketoglutarate significantly enhanced sperm viability,sperm plasma membrane integrity,plasma total antioxidant capacity,superoxide dismutase,glutathione peroxidase,and catalase activity in Hu sheep(P<0.05),and also significantly reduced plasma malondialdehyde,adrenocorticotropic hormone,interleukin-4,and tumor necrosis factor-αcontent in Hu sheep(P<0.05).Description of each supplementing with 20 g/dα-ketoglutarate can ameliorate the plasma reproductive hormone contents,antioxidant capacity,immune index contents and sperm quality of Hu sheep in autumn.

关 键 词：Α-酮戊二酸 湖羊 精子质量 抗氧化 免疫指标 

分 类 号：S816.7[农业科学—饲料科学]