北部湾植物内生真菌的分离及次级代谢产物鉴定  

Isolation and secondary metabolites identification of endophytic fungi from plants in the Beibu Gulf

作　　者：王诗怡 王玉妃 刘江晔 刘硕 李子林 刘金燕 孔凡栋 王聪 Wang Shiyi;Wang Yufei;Liu Jiangye;Liu Shuo;Li Zilin;Liu Jinyan;Kong Fandong;Wang Cong(Key Laboratory of Chemistry and Engineering of Forest Products,State Ethnic Affairs Commission,Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products,Key Laboratory of universities in Guangxi for Excavation and Development of Ancient Ethnomedicinal Recipes,Guangxi Minzu University,Nanning 530006)

机构地区：[1]广西民族大学化学化工学院,林产化学与工程国家民委重点实验室,广西林产化学与工程重点实验室/协同创新中心,广西高校民族医学古方发掘与开发重点实验室,广西民族大学,南宁530006

出　　处：《中国抗生素杂志》2024年第5期510-518,共9页Chinese Journal of Antibiotics

基　　金：广西科技基地和人才专项(No.桂科AD20297036);广西自然科学基金(No.2021GXNSFBA075036);广西民族大学相思湖青年创新团队项目(No.2021RSCXSHQN01);广西高等学校千名中青年骨干教师培育计划;广西研究生教育创新计划项目(No.YCSW2023255)。

摘　　要：目的 对北部湾3株药用植物的内生真菌进行研究,研究小叶海金沙内生真菌Spegazzinia sp.MDCW-573次级代谢产物。方法 通过10倍稀释法分离小叶海金沙、野牡丹和莲子草的内生真菌,对小叶海金沙内生真菌Spegazzinia sp.MDCW-573的次级代谢产物进行分离纯化,通过NMR、MS、ECD等方法进行结构鉴定,采用DPPH法进行化合物抗氧化活性检测,采用微量二倍稀释法进行抑菌活性测试。结果 从3株植物中分离到内生真菌171株,从Spegazzinia sp.MDCW-573的次级代谢产物中共分离得到异香豆素类化合物3个,其中化合物1为新化合物。化合物1对金黄色葡萄球菌及MRSA、化合物3对MRSA的抑菌活性MIC均为128μg/mL。结论 化合物1~3都是首次从斯氏霉属真菌的次级代谢产物分离获得,化合物1是1个新化合物,化合物1和3具有抑菌活性。Objective The endophytic fungi of three medicinal plants in the Beibu Gulf were studied,and the secondary metabolites of Spegazzinia sp.MDCW-573 from Lygodium microphyllum(Cav.) R.Br.were also investigated.Methods The endophytic fungi Lygodium microphyllum(Cav.) R.Br.,Melastoma candidum D.Don and Alternanthera sessilis(L.) R.Br.ex DC.were isolated by the ten-fold dilution method.The secondary metabolites of Spegazzinia sp.MDCW-573 from Lygodium microphyllum(Cav.) R.Br.were isolated and purified.The structures were identified by NMR,MS and ECD methods.The antioxidant activity of the compounds was detected by the DPPH method,and the antibacterial activity was tested by the micro-double dilution method.Results A total of 171 strains of endophytic fungi were isolated from these three plants.Three isocoumarin compounds were isolated from the secondary metabolites of Spegazzinia sp.MDCW-573,among which compound 1 was a new compound.Compounds 1 and 3 displayed antibacterial activitiesagainst MRSA with the same MIC value of 128 μg/mL.The MIC of compound 1 against Staphylococcus aureus was 128 μg/mL.Conclusion Compounds 1~3 were isolated from the secondary metabolites of Spegazzinia sp.for the first time.Compound 1 is a new compound,and compounds 1 and 3showed antibacterial activity.

关 键 词：广西北部湾 内生真菌 Spegazzinia sp. 次级代谢产物 

分 类 号：R978.1[医药卫生—药品]