石仙桃中4种转录因子鉴定及其组织表达的研究  

Identification and Tissue Expression of Four Transcription Factors in Pholidota chinensis

作　　者：刘保财[1,2] 张武君[1,2] 赵云青[1,2] 黄颖桢[1,2] 陈菁瑛[1,2] LIU Bao-cai;ZHANG Wu-jun;ZHAO Yun-qing;HUANG Ying-zhen;CHEN Jing-ying(Institute of Crop Sciences,Fujian Academy of Agricultural Sciences,Fuzhou 250013;Research Center for Medicinal Plant,Fujian Academy of Agricultural Sciences,Fuzhou 250013)

机构地区：[1]福建省农业科学院作物研究所,福州250013 [2]福建省农业科学院药用植物研究中心,福州250013

出　　处：《生物技术通报》2024年第5期141-152,共12页Biotechnology Bulletin

基　　金：福建省属公益类项目(2020R1034003);福建省人民政府协同创新工程项目(XTCXGC2021003);福建省农业科学院科技创新团队(CXTD2021014-2);“一带一路”国家传统草药实物库及图片信息库建设(2018FY100702)。

摘　　要：【目的】石仙桃Pholidota chinensis Lindl为珍稀濒危的附生兰,附生较地生而言进化出了许多特殊的形态结构和生理生化特性,而转录因子对调控植物的生长发育具有作用,旨在探讨和发掘石仙桃中调控其生长发育的转录因子。【方法】利用PacBio和Illumina测序技术获得石仙桃的全长转录组数据以及在根、根状茎、假鳞茎和叶片中的基因表达量,通过进一步生信分析,探讨石仙桃4种转录因子家族成员、理化特性及其组织表达特异性,并用氯化钠(NaCl)和茉莉酸甲酯(MeJA)诱导逆境胁迫,用RT-qPCR分析了C2H2家族和bHLH家族的表达量。【结果】石仙桃中鉴定的4种转录因子及其家族成员转录本为:C2H2家族21个、bHLH家族27个、WRKY家族30个、bZIP家族19个,这4个家族及其成员主要为亲水的不稳定性蛋白,亚细胞定位主要位于细胞核内,bHLH家族的部分成员位于细胞质、叶绿体等细胞器中,各基因家族均具有典型的保守结构域特征,在石仙桃的根、根状茎、假鳞茎和叶片呈现出明显的组织特异性。21个C2H2家族和27个bHLH家族中所有基因表达均受NaCl和MeJA影响,但家族成员受到促进或抑制表达及其表达量存在差异。【结论】鉴定了石仙桃中4个转录因子家族成员的数量,各家族成员主要是位于细胞核内的亲水不稳定性蛋白,并具有保守结构域特征和组织表达特异性。【Objective】Pholidota chinensis Lindl is a rare and endangered epiphytic orchid.The epiphytes have evolved many special morphological structures and physiological and biochemical characteristics compared to geophytes.The transcription factors play a role in regulating plant growth and development.The aim of this paper is to explore and discover transcription factors in P.chinensis that regulate its growth and development.【Method】This study used PacBio and Illumina sequencing techniques to obtain the full-length transcriptome data of P.chinensis and the gene expressions in the roots,rhizomes,pseudobulbs and leaves.After further bioinformatics analysis,the four transcription factor family members,physicochemical properties and tissue expression specificity of P.chinensis were revealed.The expression of C2H2 family and bHLH family was analyzed by RT-qPCR using sodium chloride(NaCl)and methyl jasmonate(MeJA)induced adversity stresses.【Result】The four identified transcription factors and their families members in P.chinensis were:21 members in C2H2 family,27 members in bHLH family,30 members in WRKY family,and 19 members in bZIP family.These four families and their members were mainly hydrophilic unstable proteins,and their subcellular localization was mainly located in the nucleus.Some members of bHLH families were located in cytoplasm,chloroplast and other organelles.The gene family had typical conserved domain characteristics,and showed obvious tissue specificity in the roots,rhizomes,pseudobulbs and leaves of P.chinensis.Expressions of all genes in C2H2 families and bHLH families were affected by NaCl and MeJA,but family members differed in whether they were promoted or repressed in the expression and in the amount of their expression.【Conclusion】The number of four transcription factor family members in P.chinensis was identified,and the members of each family are mainly hydrophilic unstable proteins located in the nucleus with conserved structural domain features and tissue expression specificity.

关 键 词：石仙桃 C2H2 BHLH WRKY BZIP 理化特性 组织表达 

分 类 号：Q94[生物学—植物学]