草珊瑚中类胡萝卜素合成的内源激素调控机制分析  

Analysis of Endogenous Hormone Regulation Mechanism for Carotenoid Synthesis in Sarcandra glabra

作　　者：吴迪 游小凤 郑亦铮 林楠 张燕燕 魏艺聪[1] WU Di;YOU Xiao-feng;ZHENG Yi-zheng;LIN Nan;ZHANG Yan-yan;WEI Yi-cong(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122)

机构地区：[1]福建中医药大学药学院,福州350122

出　　处：《生物技术通报》2024年第5期203-214,共12页Biotechnology Bulletin

基　　金：福建省自然基金面上项目(2020J01732)。

摘　　要：【目的】为了探究草珊瑚[Sarcandra glabra(thunb)nakai]不同器官中内源激素对类胡萝卜素差异积累的可能调控机制。【方法】通过代谢组学方法分析草珊瑚中内源激素和类胡萝卜素代谢物在不同器官间的差异分布情况,结合转录组学技术挖掘草珊瑚类胡萝卜素相关差异酶基因,进一步预测参与调控差异酶基因的转录因子及其激素相关顺式响应元件,从而分析内源激素对类胡萝卜素差异积累的可能调控作用。【结果】吲哚-3-羧酸(indole-3-carboxylic acid)、反式茉莉酸[(-)-jasmonic acid]、二氢茉莉酮酸甲酯(ethyl dihydrojasmonate)、油菜素甾酮(castasterone)、油菜素内酯(brassinolide)等7种内源激素在叶中的含量更高,与角黄素(canthaxanthin)、海胆酮(echinenone)、新黄质(neoxanthin)和念珠藻黄素(nostoxanthin)等8种差异代谢物等富集部位一致;而脱落酸(abscisic acid,ABA)、赤霉素4,5-甲氧基水杨酸(gibberellin 4,5-methoxysalicylic acid)、二氢茉莉酸(dihydrojasmonic acid)和5,6-二羟基吲哚(5,6-dihydroxyindole)在根中的含量更高,与脱落酸醛(abscisic aldehyde)、4,4'-二聚戊烯(4,4'-aiapolycopenedial)、花药黄质(antheraxanthin)这3种差异代谢物富集部位一致;关键转录因子MYB106、SPL1、NAC015、ERF064、WRKY44、BHLH116可通过响应AUX、SA、GA、ABA、Me_JA等植物激素的刺激,参与调控类胡萝卜素合成途径的DXS、VDE、ABA2、AOG、ZEP、CYP97C1、DWARF27、CRTISO、LCYB、PSY这10种代谢酶的表达。实时荧光定量PCR(quantitative real-time polymerase chain reaction,RT-qPCR)结果表明,随机选取的8个差异基因表达趋势与转录组测序结果一致。【结论】筛选出草珊瑚叶和根与类胡萝卜素相关的15 种差异内源激素与 11 种差异代谢物,预测了6种转录因子参与调控10 种代谢酶。【Objective】This work aims to explore the possible regulatory mechanism of endogenous hormones on the differential accumulation of carotenoids in different organs of Sarcandra glabra.【Method】In this study,metabolomics was used to analyze the differential distribution of endogenous hormones and carotenoid metabolites in different organs of S.glabra.Combined with transcriptomics technology,the differential enzyme genes related to carotenoids in S.glabra were excavated,and the transcription factors involved in the regulation of differential enzyme genes and their hormone-related cis-response elements were further predicted,so as to analyze the possible regulation of endogenous hormones on the differential accumulation of carotenoids.【Result】The contents of seven endogenous hormones such as indole-3-carboxylic acid,[(-)-jasmonic acid],ethyl dihydrojasmonate,castasterone and brassinolide were high in the leaves.Consistent with the enrichment sites of 8 differential metabolites,such as canthaxanthin,echinenone,neoxanthin and nostoxanthin;and abscisic acid,gibberellin 4,5-methoxysalicylic acid,dihydrojasmonic acid and 5,6-dihydroxyindole was high in the roots,it was consistent with the accumulation site of 3 differential metabolites,abscisic aldehyde,4,4'-aiapolycopenedial and antheraxanthin.The key transcription factor MYB106,SPL1,NAC015,ERF064,WRKY44 and BHLH116 responded to the stimulation of plant hormones such as AUX,SA,GA,ABA,and Me_JA,and participate in the regulation of carotenoid synthesis pathway DXS,VDE,ABA2,AOG,ZEP,CYP97C1,DWARF27,CRTISO,LCYB expression of these 9 metabolic enzymes.Quantitative real-time polymerase chain reaction(RT-qPCR)results showed that the expression trends of 8 randomly selected differentially expressed genes were consistent with the transcriptome sequencing results.【Conclusion】The 15 differential endogenous hormones and 11 differential metabolites related to carotenoids in the leaves and roots of Sarcandra glabra were screened,and 6 transcription factors were predicted

关 键 词：草珊瑚 类胡萝卜素 转录组 代谢组 

分 类 号：R28[医药卫生—中药学]