H5Nx禽流感病毒交叉反应性CD4^(+) T细胞表位预测  

Prediction of H5Nx Avian Influenza Virus Cross-reactivity CD4^(+)T-cell Epitopes

作　　者：谢爽 陈瑶 黄俊琼 XIE Shuang;CHEN Yao;HUANG Jun-qiong(Department of Laboratory Medicine,Affiliated Hospital of Zunyi Medical University,Zunyi 563000;Laboratory Department,Longchang People's Hospital,Longchang 642150)

机构地区：[1]遵义医科大学检验医学院,遵义563000 [2]隆昌市人民医院检验科,隆昌642150

出　　处：《生物技术通报》2024年第5期300-309,共10页Biotechnology Bulletin

基　　金：国家自然科学基金项目(81860376);遵义市科学技术基金项目(遵市科合HZ字(2021)41号)。

摘　　要：【目的】评估预先存在的H5Nx交叉反应性免疫记忆,为人类H5禽流感病毒的防治和广谱疫苗的研发提供理论数据。【方法】利用生物信息学方法筛选人源H5Nx禽流感病毒与季节性流感病毒的共同保守的交叉反应性CD4^(+) T细胞表位,进一步分析保守表位嵌套CD8^(+) T细胞表位和B细胞表位情况以及在中国人群中的覆盖率。【结果】92.3%(513/555)的H5Nx CD4^(+) T细胞表位在季节性甲型流感病毒中具有交叉反应性,其中172个表位嵌套CD8^(+) T细胞表位和5个表位嵌套B细胞表位。这些表位与相应HLA-DRB1等位基因在全世界人群中的覆盖率为88.65%。【结论】由于反复接触季节性甲型流感病毒,人群中存在一定水平的的H5Nx交叉反应性免疫记忆,能够为H5Nx感染提供部分保护。【Objective】To evaluate preexisting H5Nx cross-reactivity immune memory and to provide theoretical data for the prevention and treatment of human H5 avian influenza viruses and the development of broad-spectrum vaccines.【Method】Bioinformatics methods were used to screen the common conserved cross-reactivity CD4^(+)T cell epitopes of human H5Nx avian influenza viruses and seasonal influenza viruses,and further analysis of the nested CD8+T cell epitopes and B cell epitopes of conserved epitopes and their coverage among the Chinese population.【Result】92.3%(513/555)of H5Nx CD4^(+)T cell epitopes were cross-reactivity in seasonal influenza A viruses,of which 172 epitopes nested CD8+T cell epitopes and 5 epitopes nested B cell epitopes.These epitopes and the corresponding HLA-DRB1 alleles have a strong coverage rate of 88.65%in the world population.【Conclusion】Due to repeated exposure to seasonal influenza A viruses,there is a certain level of H5Nx cross-reactivity immune memory in the population that may provide partial protection from H5Nx infection.

关 键 词：H5Nx流感病毒 共同保守 交叉反应性 CD4^(+)T细胞表位 嵌套表位 

分 类 号：S85[农业科学—兽医学]