转录因子在微生物细胞工厂构建中的应用  

Application of transcription factors in the construction of microbial cell factories

作　　者：史彩芳 李敬知 孟栋 李春 王颖 SHI Caifang;LI Jingzhi;MENG Dong;LI Chun;WANG Ying(Key Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering of the Ministry of Industry and Information Technology,Institute of Biochemical Engineering,School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 100081,China;Key Laboratory for Industrial Biocatalysis of the Ministry of Education,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China)

机构地区：[1]北京理工大学化学与化工学院生物化工研究所医药分子科学与制剂工程工信部重点实验室,北京100081 [2]清华大学化学工程系工业生物催化教育部重点实验室,北京100084

出　　处：《生物加工过程》2024年第3期237-250,共14页Chinese Journal of Bioprocess Engineering

基　　金：国家重点研发计划(2019YFA0905700);国家自然科学基金(22078020)。

摘　　要：利用微生物作为底盘构建细胞工厂进行绿色可持续的生产已经成为当前备受关注的新型环保生产方式。随着合成生物学的发展,微生物细胞工厂的高效、“智能化”生产成为新的研究热点。微生物高效合成目的产物离不开代谢途径的调控与优化,而转录因子作为常见的调控元件,在构建微生物细胞工厂中发挥着重要的作用。同时,转录因子在生物传感器、高通量筛选及底盘细胞改造等方面有着广泛的应用。基于此,从转录因子的调控机制出发,本文综述了转录因子的挖掘、预测与鉴定方法,并对转录因子在微生物细胞工厂构建中的应用进行全面的总结与讨论,以期为相关的研究提供参考。The employment of microbial cell factories has become a feasible approach for the green and sustainable production,which is environmentally friendly and attracts much attention.With the development of synthetic biology,the efficient and intelligent production of microbial cell factories has become a topic of tremendous research hot spot.The efficient synthesis of target products in microorganisms relies on the regulation and optimization of metabolic pathways.Meanwhile,transcription factors are common regulatory elements that play an important role in the construction of microbial cell factories.Transcription factors has been widely applied in the construction of biosensors,high-throughput screening,and the engineering of chassis cells.Starting from the regulatory mechanism of transcription factors,we review the mining,prediction and identification methods of transcription factors,and comprehensively summarize and discusse the application of transcription factors in the construction of microbial cell factories to provide reference for related research.

关 键 词：转录因子 调控元件 转录调控 微生物细胞工厂 生物制造 

分 类 号：Q819[生物学—生物工程]