脐橙采后草酸浸泡处理抑制指状青霉侵染的效应  

Inhibitory effect of oxalic acid soaking treatment on Penicillium digitatum infection in postharvest navel oranges

作　　者：张洁茹 杨航云 甘崧佑 董文娟 曾教科 向妙莲[1] 陈金印[1] 陈明[1] ZHANG Jieru;YANG Hangyun;GAN Songyou;DONG Wenjuan;ZENG Jiaoke;XIANG Miaolian;CHEN Jinyin;CHEN Ming(College of Agronomy,Jiangxi Agricultural University/Jiangxi Provincial Key Laboratory for Fruits&Vegetable Storage and Preservation,Nanchang,Jiangxi,330045,China)

机构地区：[1]江西农业大学农学院/果蔬贮藏与保鲜江西省重点实验室,南昌330045

出　　处：《中国南方果树》2024年第3期59-65,共7页South China Fruits

基　　金：国家自然科学基金(32260793);江西省柑桔产业技术体系(JXARS-07)资助

摘　　要：为研究外源草酸诱导脐橙果实抗采后绿霉病的效应和抗氧化能力之间的关系,并筛选出外源草酸处理适宜浓度,以八九成成熟的“龙回红”脐橙果实为试验材料,分别使用0(清水对照)、12.5、25.0、50.0 mmol·L^(-1)草酸浸泡10 min后,在25℃环境中诱导抗性48 h,再刺伤接种指状青霉(Penicillium digitatum)孢子悬浮液,通过分析病斑直径筛选出外源草酸处理的最适浓度,并测定果实经最适浓度草酸处理和接种孢子悬浮液后黄皮层总酚和类黄酮含量、抗氧化酶活性和丙二醛(Malonaldehyde,MDA)含量及其动态变化。结果表明,12.5、25.0、50.0 mmol·L^(-1)草酸处理诱导脐橙抗绿霉病的效果分别为16.26%、25.22%和17.36%,25.0 mmol·L^(-1)的诱导效果显著高于其他浓度。25.0 mmol·L^(-1)草酸处理可以提高果实黄皮层抗坏血酸过氧化物酶、过氧化物酶和多酚氧化酶活性,增加总酚和类黄酮积累,提高自由基清除能力,抑制MDA积累,从而减轻脐橙绿霉病的发生。25.0 mmol·L^(-1)草酸处理提高脐橙对采后绿霉病的抗性,可能与其诱导提高了脐橙抗氧化能力密切相关。To investigate the relationship between the induction effect of exogenous oxalic acid(OA)on the resistance to postharvest green mold and antioxidant capacity of navel orange fruits,and screen the appropriate concentration of exogenous OA treatment,Longhuihong navel orange fruits were used as the experimental materials and soaked with 0(water treatment as control),12.5,25.0 and 50.0 mmol·L^(-1) OA for 10 min,and inducing the resistance at 25°C for 48 h,then the spore suspension of Penicillium digitatum was inoculated by pinprick of the fruits.The optimum concentration of exogenous OA treatment was screened by analyzing the diameter of lesions.The total content of phenols and flavonoids,antioxidant enzymes activities and malonaldehyde(MDA)content in flavedo and their dynamic changes in fruits treated with the optimum OA concentration and inoculated with spore suspension were determined.The results showed that the induction effect of 12.5,25.0 and 50.0 mmol·L^(-1) OA treatments on the resistance of navel orange to green mold were 16.26%,25.22%and 17.36%,respectively.The induction effect of 25.0 mmol·L^(-1) OA treatment was significantly higher than that of the other two treatments.25.0 mmol·L^(-1) OA treatment significantly increased the activities of ascorbate peroxidase(APX),peroxidase(POD)and polyphenol oxidase(PPO),promoted the accumulation of total phenols and flavonoids,improved the ability of free radical scavenging,inhibited the accumulation of MDA in flavedo,then reduced the occurrence of green mold of navel orange.25.0 mmol·L^(-1) OA treatment increased the resistance of navel orange to postharvest green mold,which may be closely related to the increase of antioxidant capacity of navel orange induced by OA.

关 键 词：脐橙 绿霉病 草酸 诱导抗性 抗氧化 

分 类 号：S66[农业科学—果树学]