北京地区儿童网织红细胞参数参考区间的建立及验证  

Establishment and validation of reference intervals for reticulocyte parameters in children from Beijing

作　　者：金芳[1] 宋文琪[1] 彭亚光[2] 何超男 Jin Fang;Song Wenqi;Peng Yaguang;He Chaonan(Department of Clinical Laboratory,Beijing Children′s Hospital,Capital Medical University,NationalCenter for Children′s Health,Beijing 100045,China;Center for Clinical Epidemiology and Evidence-based Medicine,Beijing Children′s Hospital,Capital Medical University,National Center for Children Health,Beijing 100045,China)

机构地区：[1]国家儿童医学中心、首都医科大学附属北京儿童医院,临床检验中心,北京100045 [2]国家儿童医学中心、首都医科大学附属北京儿童医院,临床流行病与循证医学中心,北京100045

出　　处：《中华检验医学杂志》2024年第5期554-560,共7页Chinese Journal of Laboratory Medicine

基　　金：国家卫生健康委医政医管局项目(国卫医医护便函(2017)374号)。

摘　　要：目的:建立北京地区儿童网织红细胞各指标参数的参考区间。方法:收集1766名(男856名,女910名)1~18岁北京地区健康儿童,幼儿组(≥1~3岁)146名,学龄前组(>3~6岁)449名,学龄组(>6~13岁)646名,青春期组(>13~18岁)525名。采用日本SySmex XN-20(A1)全自动血细胞分析仪进行静脉血网织红细胞百分比(RET%)、网织红细胞计数绝对值(RET#)、低荧光强度网织红细胞百分比(LFR)、中荧光强度网织红细胞百分比(MFR)、高荧光强度网织红细胞百分比(HFR)、未成熟网织红细胞百分比(IRF)、网织红细胞血红蛋白含量(RET-He)7项参数的检测。对检测结果进行分析后,以百分位数(P2.5,P97.5)建立参考区间。参考区间建立后另收集109名北京地区健康体检儿童静脉血,依据《临床实验室检验项目参考区间的制定》对参考区间进行验证。结果:1~18岁儿童网织红细胞7项指标参数的参考区间无需进行年龄及性别分组,参考区间为RET%为0.74%~2.06%,RET#为(34.5~101.5)×109L,LFR为86.6%~96.5%,MFR为3.2%~11.5%,HFR为0~2.1%;IRF为3.5%~13.4%;RET-He为28.5~34.2 pg。109份验证标本检测结果在参考区间范围内>90%。结论:本研究建立了北京地区1~18岁健康儿童网织红细胞7项指标参数的参考区间,并通过参考区间验证。Objective To establish the reference intervals of reticulocyte parameters for children in the Beijing area.Methods 1766(856 males and 910 females)healthy children aged from 1 to 18 years old in the Beijing area were studied,the infant group(≥1-3 years old)included 146 participants;the preschool group(>3-6 years old)had 449 participants;the school age group(>6-13 years old)had 646 participants and the adolescent group(>13-18 years old)had 525 participants.Seven parameters were measured in venous blood by SySmex XN-20(A1)automatic blood cell analyzer,which included reticulocyte percentage(RET%),absolute reticulocyte count(RET#),low fluorescence reticulocyte(LFR),medium fluorescence reticulocyte(MFR),high fluorescence reticulocyte(HFR),immature reticulocyte fraction(IRF)and reticulocyte hemoglobin equivalent(RET-He).After the test results were collected,the reference intervals were established according to the percentile(P2.5,P97.5).As the reference intervals were established,venous blood samples from 109 healthy children in Beijing were collected to verify the reference intervals according to WS/T 402-2012"Define and determine the reference intervals in clinical laboratory".Results The reference interval of 7 Reticulocyte parameters for children aged 1-18 years without age and sex grouping,reference interval(RET%):0.74%-2.06%;absolute reticulocyte count(RET#):(34.5-101.5)×109/L;low fluorescence reticulocyte(LFR):86.6%-96.5%;medium fluorescence reticulocyte(MFR):3.2%-11.5%;high fluorescence reticulocyte(HFR):0%-2.1%;immature reticulocyte fraction(IRF):3.5%-13.4%;reticulocyte hemoglobin equivalent(RET-He):28.5-34.2 pg.Over 90%test results of samples from the 109 children ranged within the reference ranges.Conclusion This study established convincible reference intervals of seven reticulocyte parameters for healthy children aged from 1 to 18 in the Beijing area was established,and reference interval verification passed.

关 键 词：儿童 网织红细胞 网织红细胞参数 参考区间 

分 类 号：R725[医药卫生—儿科] R446.1[医药卫生—临床医学]