两种寄主植物对淡足侧沟茧蜂的引诱作用及挥发物成分分析  

Attractiveness of two host plants to Microplitis pallidipes and their volatile compounds identification

作　　者：张彤瑶 蒋杰贤 张浩 王金彦 尤春梅 陈义娟 季香云 ZHANG Tong-Yao;JIANG Jie-Xian;ZHANG Hao;WANG Jin-Yan;YOU Chun-Mei;CHEN Yi-Juan;JI Xiang-Yun(College of Life Science and Fishery,Shanghai Ocean University,Shanghai 201306,China;Shanghai Runzhuang Agricultural Technology Co.,Ltd.Shanghai 201403,China;Eco-environment Protection Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海润庄农业科技有限公司,上海201403 [3]上海市农业科学院生态环境保护研究所,上海201403

出　　处：《应用昆虫学报》2024年第1期62-73,共12页Chinese Journal of Applied Entomology

基　　金：上海市青年科技启明星计划(21QB1404100);上海市农业科学院卓越创新团队[2022(017)];上海市青年科技英才扬帆计划(21YF1441100)。

摘　　要：【目的】为了更好的了解寄主植物和甜菜夜蛾Spodoptera exigua幼虫对淡足侧沟茧蜂Microplitis pallidipes趋向行为的影响。【方法】本研究选用玉米和苋菜两种寄主植物,采用“Y”型嗅觉仪测定了淡足侧沟茧蜂对正常植物和幼虫-植物复合体的行为反应,并对其释放的挥发性物质进行了GC-MS分析与鉴定。【结果】正常的玉米和苋菜对淡足侧沟茧蜂无明显引诱作用,而幼虫-玉米复合体和幼虫-苋菜复合体均有显著的吸引作用,且幼虫-玉米复合体对该寄生蜂的引诱作用更强。GC-MS检测结果显示在正常玉米中含有22种化合物,主要为烷烃和芳香烃类化合物;幼虫-玉米复合体中有31种化合物,以烷烃和醇类化合物为主;在正常苋菜检测到的16种化合物中,烷烃类化合物含量最高,约占54.75%;而幼虫-苋菜复合体中含有55种化合物,其中以烷烃、芳香烃、烯烃、酸类和醛类物质含量相对较高。另外,与正常植物相比,2-己烯醛、(+)-柠檬烯、α-石竹烯、DMNT和α-雪松烯等化合物仅在幼虫-植物复合体中产生或含量明显升高。【结论】淡足侧沟茧蜂更倾向于幼虫-玉米复合体,并且玉米和苋菜在遭受甜菜夜蛾取食后,会释放出更多种类的化合物。[Aim]To better understand the effect of host plants and Spodoptera exigua on the tendency behavior of Microplitis pallidipes.[Methods]Maize and amaranth were chosen and the behavioral responses of M.pallidipes to normal plants and larva-plant complex were determined with Y-tube olfactometer,and the volatile compounds were analyzed and identified by GC-MS.[Results]The normal maize and amaranth had no attraction effect on M.pallidipes,but larva-maize complex and larva-amaranth complex exhibited significant attractiveness,and larva-maize complex showed stronger activity than larva-amaranth complex.GC-MS indicated that 22 compounds were detected in normal maize leaves with alkanes and aromatic hydrocarbons as the main constitutes,while 31 compounds were identified in the larva-maize complex and mainly alkanes and alcohols.Similarly,there were 16 compounds in normal amaranth and the content of alkane compounds was the highest with 54.75%.The larva-amaranth complex contained 55 compounds,and alkanes,aromatics,alkenes,acids and aldehydes were relatively high.In addition,some compounds were only produced or increased remarkably in larva-plant complex,such as 2-hexenal,(+)-limonene,α-caryophyllene,DMNT,andα-himachalene.[Conclusion]M.pallidipes were prefer the larva-maize complex,and a wider variety of compounds were released by maize and amaranth after feeding by Spodoptera exigua.

关 键 词：淡足侧沟茧蜂 玉米 苋菜 甜菜夜蛾 引诱作用 GC-MS 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]