越冬滞育前后松阿扁叶蜂幼虫体壁防御体系的变化  

Diapause-induced changes in cuticle defense compounds in Acantholyda posticalis larvae

作　　者：王海香[1] 陈凡 师振萍 薛婉怡 孙亚星 高瑞贺 郭红彦[1] WANG Hai-Xiang;CHEN Fan;SHI Zhen-Ping;XUE Wan-Yi;SUN Ya-Xing;GAO Rui-He;Guo Hong-Yan(Forestry College,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学林学院,太谷030801

出　　处：《应用昆虫学报》2024年第1期74-81,共8页Chinese Journal of Applied Entomology

基　　金：山西省青年基金(20210302124359)。

摘　　要：【目的】食叶害虫松阿扁叶蜂Acantholyda posticalis Matsumura以老熟幼虫在地下越冬,越冬期间体壁保护其不受外来物的侵害。研究松阿扁叶蜂幼虫体壁对生态的适应性,对于其管理和防治具有重要意义。【方法】采用石油醚溶解脂肪、饱和KOH消化几丁质和考马斯亮蓝结合法测定松阿扁叶蜂不同龄期幼虫蛋白质质量和含量;采用FB 28和HE染色法研究幼虫体壁几丁质和脂肪发育的动态变化;采用紫外分光光度计比色法测定体壁多酚氧化酶的蛋白含量和活性变化。【结果】(1)松阿扁叶蜂幼虫体壁几丁质、总蛋白质、总脂肪含量均随着幼虫发育阶段的变化而增加:蛹前滞育>入土滞育>5龄>4龄,且在5龄至入土前迅速增长。(2)松阿扁叶蜂幼虫体壁的几丁质和脂肪厚度随幼虫发育阶段变化趋势为:入土滞育>蛹前滞育>5龄>4龄,且在5龄至入土前迅速增长。(3)随着幼虫发育,松阿扁叶蜂幼虫总脂肪含量变化平缓,几丁质含量呈现下降-升高-下降趋势,总蛋白质含量呈现升高-降低-升高趋势。(4)随着幼虫的发育,松阿扁叶蜂幼虫体壁多酚氧化酶含量呈现先升高后持续降低的趋势,而活力正好相反,变化波形趋势近似互补。【结论】几丁质和蛋白质含量变化曲线相反,波形互补,多酚氧化酶的含量-活性变化互补,这两种互补在松阿扁叶蜂越冬滞育和非滞育期间又构成了互补的体壁防御体系。[Aim]Acantholyda posticalis Matsumura is a defoliating insect.The cuticle of mature A.posticalis larva protects it from the external environment during overwintering.Understanding the ecological adaptability of the larval cuticle is essential for the integrated management of A.posticalis.[Methods]Variability in the quality and quantity of chitin,fats,and protein in the larval cuticle were investigated by dissolving fat in mineral ether,digesting chitin using saturated KOH,and detecting protein with Coomassie brilliant blue.Variation in the dynamic development of chitin and fats was investigated using Fluorescent Brightener 28(FB28)and Hematoxylin-eosin staining(HE).Colorimetry was used to determine the protein content and activity of polyphenol oxidase(PPO on the integument.[Results](1)The quality of chitin,total protein,and total fats in the cuticle gradually increased from prepupal diapause,through the soil diapause,the 5th instar and the 4th instar larva stages.There was a marked increase from the fifth instar to the point of entering the soil.(2)Chitin and fat thickness of the larval integument varied during development from soil diapause through to prepupal diapause,the 5th instar,and 4th instar stages.Thickness increased rapidly from the 5th instar stage to entering the soil.(3)There was minimal variation in total fat content,whereas chitin content showed a decreasing-increasing-decreasing trend.Total protein content also showed an increasing-decreasing-increasing trend during larval development.(4)The variation in protein content and PPO activity showed a complementarity trend.As the larvae developed,the protein content of PPO in the larval integument initially increased,then showed a consistent decrease.In contrast,PPO activity displayed the opposite trend.[Conclusion]The variation in chitin and protein content of larval integument exhibited an opposing but complementary pattern.The fluctuations in protein content and PPO activity appeared to be complimentary.These two forms of complimentary variation col

关 键 词：松阿扁叶蜂 滞育 体壁防御 化学成分 多酚氧化酶 

分 类 号：S763.43[农业科学—森林保护学]