自拟癣良方对咪喹莫特诱导小鼠银屑病的治疗作用及机制研究  

Therapeutic effects and mechanism of a self-formulated Chinese herbal formula for psoriasis induced by imiquimod in mice

作　　者：李金隆 曹德森[2] 曹岗[1] LI Jinglong;CAO Desen;CAO Gang(College of Pharmacy,Zhejiang Chinese Medical University,Hangzhou 310053,China;Department of Medical Equipment Engineering,Chinese People's Liberation Army General Hospital,Beijing 100853,China)

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]解放军总医院医学工程科,北京100853

出　　处：《军事医学》2024年第3期219-224,共6页Military Medical Sciences

摘　　要：目的观察自拟癣良方对咪喹莫特诱导小鼠类银屑病的治疗作用并探讨其作用机制。方法BALB/c小鼠随机分为正常组、模型组及口服外涂组,每组10只。采用背部涂布5%咪喹莫特乳膏诱导建立BALB/c小鼠银屑病模型,考察自拟癣良方对银屑病的治疗作用。于咪喹莫特乳膏诱导前,以及诱导后第3、7、10及12天,通过光声成像技术,观察小鼠皮肤血管分布和迂曲变化,评价自拟癣良方对血管异常新生的影响。采用苏木素-伊红染色,评价实验小鼠皮肤局部病理变化。采用酶联免疫吸附试验检测小鼠皮肤组织及血清中肿瘤坏死因子α(TNF-α)、白细胞介素-17(IL-17)及白细胞介素-6(IL-6)等细胞因子含量变化。通过流式细胞术,考察小鼠脾脏中Treg、Th1、Th17等细胞因子含量。结果与模型组相比,口服外涂组银屑病面积与严重性指数(PASI)评分显著下降(P<0.01),口服外涂组皮肤中TNF-α、IL-17及IL-6含量显著下调(P<0.01),口服外涂组Treg、Th17及Th1细胞比例明显下降(P<0.05),口服外涂组小鼠皮肤组织病理学(Baker法)评分明显降低(P<0.05)。结论自拟癣良方对银屑病具有良好治疗作用,可通过免疫抑制作用抑制银屑病皮损。Objective To observe the therapeutic effect of a self-formulated formula for psoriasis on imiquimod induced BALB/c mouse psoriasis model and explore its mechanism of action.Methods The BALB/c mice were randomly divided into the normal group,model group,and oral external group(all n=10).The BALB/c mouse psoriasis like model was induced by using back application of 5%imiquimod cream to verify the therapeutic effect of the self-formulated formula for psoriasis.Before induction with imiquimod cream,as well as on the 3rd,7th,10th,and 12th day after induction,the distribution and tortuous changes of blood vessels in the skin of mice were observed by using photoacoustic imaging technology to evaluate the effect of the formula on abnormal angiogenesis.The local pathological changes in the skin of experimental mice wasevaluated by using the Hematoxylin-Eosin staining.The enzyme linked immunosorbent assay was used to detect changes in the content of cytokines such as tumor necrosis factor(TNF-α),interleukin-17(IL-17),and interleukin-6(IL-6)in mouse skin tissue and serum.By using the flow cytometry,the levels of cytokines such as Treg,Th1,and Th17 in mouse spleen were investigated.Results Compared with the model group,the area and severity index score of psoriasis(PASI)in the oral external group was significantly decreased(P<0.01),and the levels of TNF-α,IL-17,and IL-6 in the skin of the oral external group were significantly down regulated(P<0.01).Compared with the model group,the proportion of Treg,Th17,and Th1 cells in the oral external group was significantly reduced(P<0.05),and the pathological scores of skin tissue(Baker's method)in the oral external group were significantly reduced(P<0.05).Conclusion Self-formulated formula for psoriasis has a good therapeutic effect on psoriasis and can suppress psoriasis skin lesions through immunosuppressive effects.

关 键 词：银屑病 小鼠 中药 光声成像 免疫抑制 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]