Electrobiocorrosion by microbes without outer-surface cytochromes  

作　　者：Dawn E.Holmes Trevor L.Woodard Jessica A.Smith Florin Musat Derek R.Lovley 

机构地区：[1]Department of Microbiology,University of Massachusetts Amherst,Amherst,Massachusetts,USA [2]Department of Physical and Biological Science,Western New England University,Springfield,Massachusetts,USA [3]Department of Biomolecular Sciences,Central Connecticut State University,New Britain,Connecticut,USA [4]Department of Biology,Section for Microbiology,Aarhus University,Aarhus,Denmark [5]Department of Molecular Biology and Biotechnology,Facuity of Biology and Geology,Babes-Bolyai University,Cluj-Napoca,Romania

出　　处：《mLife》2024年第1期110-118,共9页微生物（英文）

摘　　要：Anaerobic microbial corrosion of iron-containing metals causes extensive economic damage.Some microbes are capable of direct metal-to-microbe electron transfer(electrobiocorrosion),but the prevalence of electrobiocorrosion among diverse methanogens and acetogens is poorly understood because of a lack of tools for their genetic manipulation.Previous studies have suggested that respiration with 316L stainless steel as the electron donor is indicative of electrobiocorrosion,because,unlike pure Fe^(0),316L stainless steel does not abiotically generate H_(2) as an intermediary electron carrier.Here,we report that all of the methanogens(Methanosarcina vacuolata,Methanothrix soehngenii,and Methanobacterium strain IM1)and acetogens(Sporomusa ovata and Clostridium ljungdahli)evaluated respired with pure Fe^(0)as the electron donor,but only M.vacuolata,Mx.soehngeni,and S.ovata were capable of stainless steel electrobiocorrosion.The electrobiocorrosive methanogens re-quired acetate as an additional energy source in order to produce methane from stainless steel.Cocultures of S.ovata and Mx.soehngeni demonstrated how acetogens can provide acetate to methanogens during corrosion.Not only was Meth-anobacterium strain IM1 not capable of electrobiocorrosion,but it also did not accept electrons from Geobacter metal-lireducens,an effective electron-donating partner for direct interspecies electron transfer to all methanogens that can directly accept electrons from Fe^(0).The finding that M.vacuolata,Mx.soehngeni,and S.ovata are capable of electrobiocorrosion,despite a lack of the outer-surface c-type cytochromes previously found to be important in other electrobiocorrosive microbes,demonstrates that there are multiple microbial strategies for making electrical contact with Fe^(0).

关 键 词：ACETOGEN CORROSION Fe^(0) METHANOGEN stainless steel 

分 类 号：Q93[生物学—微生物学]