新型喹啉基铜配合物抑制HeLa细胞的增殖、迁移和侵袭  

Inhibition of Proliferation,Migration and Invasion of HeLa Cells by Novel Quinoline Copper Complex

作　　者：贺卿 黄伟 周鑫慧 刘广欢 高春艳[1] 张永坡[1] 杨美红[1] 赵晋忠[1] 杜维俊[2] He Qing;Huang Wei;Zhou Xinhui;Liu Guanghuan;Gao Chunyan;Zhang Yongpo;Yang Meihong;Zhao Jinzhong;Du Weijun(Department of Basic Sciences,Shanxi Agricultural University,Taigu 030801,China;College of Agronomy,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学基础部,山西太谷030801 [2]山西农业大学农学院,山西太谷030801

出　　处：《南开大学学报（自然科学版）》2024年第2期56-63,共8页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：山西省重点研发计划项目(201903D221036);山西省大学生创新创业训练计划项目(20230210);山西农业大学引进人才博士科研启动项目(412576)。

摘　　要：合成了一例新的铜配合物[CuL(Cl)2(C2H5OH)](1),并用FT-IR红外和X-射线衍射对其进行了表征.采用MTT法测定配合物对HeLa、HepG-2、NCI-H460、BGC-823、HUVEC细胞的体外增殖抑制作用.结果表明该配合物对HeLa细胞具有较高的细胞毒性作用,且对正常细胞HUVEC的毒性(IC_(50)=(72.27±7.77)μmol/L)远低于奥沙利铂(IC_(50)=(10.64±3.68)μmol/L).此外,体外抗肿瘤实验表明配合物可诱导HeLa细胞凋亡、上调细胞内活性氧(ROS)水平,降低线粒体膜电位,抑制肿瘤细胞的迁移、侵袭和内皮细胞的血管生成.A new Cu(Ⅱ)complex[CuL(Cl)2(C2H5OH)](1)was synthesized and characterized by fourier transform infrared spectrum and X-ray diffraction.The MTT assay was used to determine the inhibitory effect on the proliferation of HeLa,HepG-2,NCI-H460,BGC-823 and HUVEC cells in vitro.The results showed that the complex showed high cytotoxicity to HeLa cells,and the level of toxicity to normal HUVEC cells(IC_(50)=(72.27±7.77)μmol/L)was much less than that of oxaliplatin(IC_(50)=(10.64±3.68)μmol/L).Furthermore,in vitro anti-tumor experiments showed that the complex was able to induce apoptosis of HeLa cells,up-regulating the level of intracellular ROS,decreasing mitochondrial membrane potential,inhibiting tumor cell migration and invasion,and inhibiting vascular endothelial cell formation.

关 键 词：铜配合物 抗肿瘤 侵袭 迁移 

分 类 号：O614.12[理学—无机化学]