Bioactivity of silk fibroin peptides on vascular endothelial cells  

作　　者：Mengnan Dai Meng Li Peixuan Li Boyu Zhang Jianmei Xu Jiannan Wang 

机构地区：[1]National Engineering Laboratory for Modern Silk,College of Textile and Clothing Engineering,Soochow University,No.199 Ren-ai Road,Suzhou Industrial Park,Suzhou 215123,China

出　　处：《Frontiers of Materials Science》2024年第1期85-94,共10页材料学前沿（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.51873141).

摘　　要：To determine the contribution of non-repetitive domains to the bioactivity of the heavy chain in silk fibroin(SF)macromolecules,a gene motif f(1)encoding this fragment and its multimers(f(4)and f(8))were biosynthesized from Escherichia coli BL21.Based on the positive application potential of SF materials for the vascular tissue engineering,this study focused on examining the active response of these polypeptides to vascular endothelial cells.Biosynthetic polypeptides F(1),F(4),and F(8)were separately grafted onto the surfaces of bioinert polyethylene terephthalate(PET)films,resulting in remarkable improvements in the spread and proliferation of human umbilical vein endothelial cells(HUVECs).Using the same grafting dose,the activity of cells on polypeptide-modified PET films enhanced with the increase of the molecular weight of those grafted polypeptides from F(1)to F(8).Meanwhile,the growth of cells on the surface of the alkaline-treated PET film was improved,indicating that the hydrophilicity of the surface material had influence on the growth of HUVECs.Moreover,on surfaces with the same water contact angle,the spread and proliferation activity of cells on PET films were significantly lower than those on polypeptide-modified PET films.

关 键 词：silk fibroin heavy chain non-repetitive fragment cytoactive endothelial cell 

分 类 号：R318[医药卫生—生物医学工程]