计算机显微断层扫描技术(μCT)在量化阔叶树种导管中的应用  

Application of micro-computed tomography(μCT)in quantifying xylem vessels of broadleaved trees

作　　者：白春梅 梁国芹 符韵林[1,2,3] 韩尔康 郭霞丽 王丰 BAI Chunmei;LIANG Guoqin;FU Yunlin;HAN Erkang;GUO Xiali;WANG Feng(Guangxi Key Laboratory of Forest Ecology and Conservation,College of Forestry,Guangxi University,Nanning 530004,China;Guangxi Youyiguan Forest Ecosystem National Observation and Research Station,Pingxiang 532600,Guangxi,China;Youyiguan Forest Ecosystems Observation and Research Station of Guangxi,Pingxiang 532600,Guangxi,China;Guangzhou Jingqi Rui Biotechnology Co.,Ltd,Guangzhou 510005,China;Centre Eau Terre Environnement,Institute National de la Recherche Scientifique,Québec G1K 9A9,Canada;GEOTOP Research Centre in the Dynamics of the Earth System,Montréal H2X 3Y7,Canada)

机构地区：[1]广西大学林学院,广西森林生态与保育重点实验室,南宁530004 [2]广西友谊关森林生态系统国家定位观测研究站,广西凭祥532600 [3]崇左凭祥友谊关森林生态系统广西野外科学观测研究站,广西凭祥532600 [4]广州市金圻睿生物科技有限责任公司,广州510005 [5]魁北克国立科学研究院水地环境中心,加拿大魁北克G1K 9A9 [6]GEOTOP地球系统动力学研究中心,加拿大蒙特利尔H2X 3Y7

出　　处：《应用生态学报》2024年第5期1214-1222,共9页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金青年项目(32201543);广西科技基地和人才专项(2021AC19325)资助。

摘　　要：在细胞尺度上定量分析导管特征,对于揭示植物对环境的适应策略具有重要意义。直接打磨样品并结合体视显微镜成像技术是目前研究木质部解剖结构(针叶树管胞和阔叶树导管)的主要方法之一,但仍不可避免损伤木质部细胞,限制了对木质部细胞真实解剖结构的认识。本研究选取了加拿大魁北克南部的糖枫树、北美白桦、美洲白蜡树、美洲铁木、大齿杨、苦味山核桃、北美红橡7个阔叶树种,应用计算机显微断层扫描技术(μCT)和体视显微镜法分别测量各树种的导管直径和面积,采用线性模型对两种方法的测量结果进行数据拟合,探讨采用μCT量化阔叶树导管大小的可行性。结果表明:对所测定的7个树种,应用两种方法测量导管大小的结果高度相似(R~2=0.98)。环孔材树种应用两种方法测量的导管直径结果拟合优度(苦味山核桃R~2=0.98,美洲白蜡树R~2=0.96,北美红橡R~2=0.99)高于散孔材树种(北美白桦R~2=0.88,美洲铁木R~2=0.73,糖枫树R~2=0.68,大齿杨R~2=0.88)。应用2种方法测量小导管(直径≤200μm,R~2=0.94)的结果拟合优度高于大导管(直径>200μm,R~2=0.92)。μCT技术为量化阔叶树种木质部导管提供了一种无损检测新途径。Quantitative analysis of vessel characteristics at the cellular scale is of great significance for understan-ding plant adaptation strategies to environment.The direct grinding combined with stereo-microscope imaging is one of the main approaches to examine the anatomical structure of xylem(conifer tracheid and hardwood vessel)wood structure,which inevitably damages xylem cells,hindering the accurate understanding of anatomical structures.In this study,we applied X-ray micro-computed tomography(μCT)and stereo-microscope technology to quantitatively measure the diameter and area of vessels of seven Canadian broadleaved tree species(Acer saccharum,Betula papyrifera,Fraxinus americana,Ostrya virginiana,Populus grandidentata,Quercus rubra,and Carya cordiformis).We fitted the results by linear model and tested the feasibility ofμCT technology in quantifying the vessel size of broadleaved species.We found that the results of the two methods for measuring vessel size were highly similar(R~2=0.98).The goodness of fit of the vessel diameter results measured by the two methods for the ring-porous wood species(C.cordiformis,R~2=0.98;F.americana,R~2=0.96;Q.rubra,R~2=0.99)was higher than that of the diffuse-porous wood species(B.papyrifera,R~2=0.88;O.virginiana,R~2=0.73;A.saccharum,R~2=0.68;P.grandiden-tata,R~2=0.88).The goodness of fit of small vessels(diameter≤200μm,R~2=0.94)measured by the two methods was higher than that of large vessels(diameter>200μm,R~2=0.92).Thus,theμCT technique provided a new non-destructive detection method for quantifying xylem vessels of broadleaved tree species.

关 键 词：木质部 导管 MICRO-CT 阔叶树 

分 类 号：S718.4[农业科学—林学]