天南星和虎掌南星胆汁炮制前后毒性对比研究  

Comparative Study on Toxicity of ARISAEMATIS RHIZOMA and Pinelliae Pedatisectae Schott Before and After Processing with Bile

作　　者：武旭 钟恋 王晶[1,2] 邹婷 杨凯 雷晗丹 党明 贺素容 张桥 卫培峰[4] 赵重博 WU Xu;ZHONG Lian;WANG Jing;ZOU Ting;YANG Kai;LEI Handan;DANG Ming;HE Surong;ZHANG Qiao;WEI Peifeng;ZHAO Chongbo(Shaanxi University of Chinese Medicine,Engineering Technology Research Center of Shaanxi Administration of Chinese Herbal Pieces,Xianyang 712046;Shaanxi Traditional Chinese Medicine Processing Technology Heritage Base,Xianyang 712046;Sichuan Institute for Drug Control,Chengdu 611137;Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang 712046;Xi'an Daxing Hospital,Xi'an 710000)

机构地区：[1]陕西中医药大学陕西省中药饮片工程技术研究中心,咸阳712046 [2]陕西省中药炮制技术传承基地,咸阳712046 [3]四川省药品检验研究院,成都611137 [4]陕西中医药大学第二附属医院,咸阳712046 [5]西安大兴医院,西安710000

出　　处：《中药药理与临床》2024年第3期83-91,48,共10页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金青年项目(编号:81803732);中国药品监管科学行动计划第二批重点项目(编号:NMPAJGKX-2023-077);四川省药品检验研究院院立科研项目(编号:2023-KYYL-001);四川省自然科学基金(编号:2022NSFSC1572);陕西省中医药管理局“双链融合”中青年科研创新团队项目(编号:2022-SLRH-YQ-008)。

摘　　要：目的:探讨胆汁制天南星和虎掌南星前后的急性毒性、刺激性和燥性效应变化。方法:将132只小鼠随机分组后进行急性毒性试验;39只家兔随机分组后进行兔眼刺激试验;104只大鼠随机分组进行燥性试验。以小鼠中毒表现、兔眼刺激评分和大鼠的饮水量、排尿指数、大便含水率和颌下腺、肾脏、结肠的病理变化以及水通道蛋白5(Aquaporin5,AQP5)、AQP2、AQP3的表达、血浆粘度、血清中环磷腺苷/环磷酸鸟苷(adenosine cyclic 3’,5’-hydrogen phosphate/current good manufacture practices,cAMP/cGMP)、三磷酸腺苷酶(ATPase,Na^(+)-K^(+)-ATP酶)、血浆中的肌酐(Cr)和尿素氮(BUN)含量等指标进行考察。结果:天南星和虎掌南星胆汁炮制前后的水煎液对小鼠灌胃无毒性,小鼠最大耐受量生品均为108 g/kg,炮制品均为75.6 g/kg。胆汁炮制后对家兔眼刺激的作用显著减弱(P<0.05);天南星和虎掌南星对正常大鼠结肠、颌下腺、肾脏有明显损伤作用;显著升高血浆中Cr和BUN的含量(P<0.05或P<0.01),胆汁炮制后对正常大鼠结肠、颌下腺、肾脏组织损伤作用较弱。天南星和虎掌南星可使正常大鼠血液中cAMP/cGMP、Na^(+)-K^(+)-ATP酶含量和肾脏、结肠、颌下腺组织中水通道蛋白(AQP2、AQP3、AQP5)表达异常(P<0.05或P<0.01),使正常大鼠出现水液代谢紊乱、血液黏度增加、排尿指数增加、排便量减少、大便干结(P<0.05或P<0.01),各胆汁制南星组大鼠相应的指标考察中发现与生品相比其作用较弱。结论:胆汁炮制可使天南星和虎掌南星刺激性和燥性减弱,本研究可为天南星的“炮制缓燥”和“炮制减毒”提供科学依据。Objective:To investigate the changes of acute toxicity,iriation,and dyness of ARISAEMATIS RHIZOMA and Pinelliae Pedaisectae Schott before and after processing with bile.Methods:A total of 132 mice were randomly divided.and an acute toxicity lest was performed.39 rabbits were randomly divided.and an eye stimulation test was arried out.A total of 104 rals were randomly divided to perform a dryness test.The toxic manifestation of rats in each group,iritation score of rabbit eyes,waler intake of rats.urination index,water content of stool.the pathological changes of submandibular gland,kidney,and colon,the expression levels of aquaporin 5(AQP5),aquaporin 2(AQP2),and aquaporin 3(AQP3),the plasma viscosity,and the contents of adenosine cyelic 3',5'-hydrogen phosphate/current good manufacture practices(cAMP/cGMP),ATPase(Na^(+)-K^(+)-ATPse),creatinine(Cr),and blood urea nitrogen(BUN)in plasma were determined.Results:The wa-ter decoction of ARISAEMATIS RHIZOMA and Pinelliae Pedatisetae Schotl before and after processing with bile was non-toxie for rats.The maximum tolerance dose of ARISAEMATIS RHIZOMA and Pinelliae Pedatisectae Schott for rats was 108 g/kg,and that of ARISAEMATIS RHIZOMA and Pinelliae Pedatisectae Schott after procssing with bile for rats was 75.6 g/kg.The stimulation on rabbit eyes of ARISAEMA-TIS RHIZOMA and Pielliace Pedatisectae Schott after processing with bile significantly deereased(P<0.05).The damage efeets of ARISAE.MATIS RHIZOMA and Pinelliae Pedatisectae Schott on the colon,submandibular gland,and kidney in normal mice were obvious.ARISAE.MATIS RHIZOMA and Pellice Pedulisectae Schotl sigifcanly increased the contents of Cr and BUN in plasma(P<0.05 orP<0.01).Ar.ter processing with bile,their damage efets on the colon,submandibular gland,and kidney in normal mice were llviatede.In aditin,ARISAEMATIS RHIZOMA and Pinelliae Pedatisectae Schott could make the content of cAMP/cGMP and ATPase in blood and the expression of apaporimnp(AQP,AQP3,and AQP5)in the kidney,cnlom,and submandibular gland of norm

关 键 词：天南星 虎掌南星 牛胆汁 胆南星 燥性 毒性 

分 类 号：R285.5[医药卫生—中药学]