肥胖因素对小鼠心肌缺血再灌注损伤的影响及TRPA1表达在其中的作用  

Effect of obesity factor on myocardial ischemia-reperfusion injury in mice and role of TRPA1 expression

作　　者：王敏[1] 秦帅 李傲雪 吴云[1] Wang Min;Qin Shuai;Li Aoxue;Wu Yun(Department of Anesthesiology and Perioperative Medicine,the Second Affiliated Hospital of Anhui Medical University,Key Laboratory of Anesthesiology and Perioperative Medicine of Anhui Higher Education Institutes,Anhui Medical University,Hefei 230601,China)

机构地区：[1]安徽医科大学第二附属医院麻醉与围术期医学科,麻醉与围术期医学科安徽普通高校重点实验室,合肥230601

出　　处：《中华麻醉学杂志》2024年第4期471-475,共5页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金青年科学基金项目(81900316);安徽省自然科学基金优青项目(2308085Y42);安徽省高校自然科学研究重点项目(2023AH053158)。

摘　　要：目的评价肥胖因素对小鼠心肌缺血再灌注损伤的影响及瞬时受体电位锚蛋白1(TRPA1)表达在其中的作用。方法SPF级健康雄性C57BL/6J小鼠24只,6~7周龄,体质量18~21 g。采用随机数字表法分为2组(n=12):普通饲料组(CD组)和高脂饲料组(HFD组)。CD组喂养普通饲料,HFD组喂养60%脂肪供能的高脂饲料,持续12周并每周记录小鼠体质量。随后将CD组小鼠随机分为2组(n=6):CD+溶剂组(CD+C组)和CD+AITC组(CD+A组);HFD组小鼠随机分为2组(n=6):HFD+溶剂组(HFD+C组)和HFD+TRPA1激动剂组(HFD+A组)。第13周,CD+A组和HFD+A组在原先饲料喂养基础上使用TRPA1激动剂异硫氰酸烯丙酯25 mg·kg^(-1)·d^(-1)进行灌胃,CD+C组和HFD+C组给予等量溶剂。第14周小鼠心肌缺血30 min再灌注120 min,记录小鼠体质量、附睾脂肪、肠系膜脂肪和肾周脂肪的质量,检测血清LDH、甘油三酯和胆固醇水平,计算心肌梗死体积百分比,采用Western blot法检测心肌组织TRPA1、Bax和Bcl-2的表达,计算Bax/Bcl-2比值。结果与CD+C组比较,CD+A组小鼠心肌组织TRPA1表达上调(P<0.05),其余各指标差异无统计学意义(P>0.05),HFD+C组小鼠血清甘油三酯、胆固醇浓度、体质量、附睾脂肪、肠系膜脂肪、肾周脂肪质量和心肌梗死体积百分比增加,心肌组织TRPA1表达下调,LDH浓度和Bax/Bcl-2比值升高(P<0.05);与HFD+C组相比,HFD+A组小鼠血清甘油三酯和胆固醇浓度降低,体质量、附睾脂肪、肠系膜脂肪、肾周脂肪质量和心肌梗死体积百分比减少,心肌组织TRPA1表达上调,LDH浓度和Bax/Bcl-2比值降低(P<0.05)。结论肥胖因素可加重小鼠心肌缺血再灌注损伤,TRPA1表达下调参与了这一过程。Objective To evaluate the effect of obesity factor on myocardial ischemia-reperfusion injury and the role of transient receptor potential ankyrin 1(TRPA1)expression in mice.Methods Twenty-four SPF healthy male C57BL/6J mice,aged 6-7 weeks,weighing 18-21 g,were divided into 2 groups(n=12 each)using a random number table method:common diet group(CD group)and high fat diet group(HFD group).CD group was fed a common diet and HFD group was fed a high-fat diet supplied with 60%fat for 12 weeks,and the body weight of mice was recorded every week.The mice in CD group were then divided into 2 groups(n=6 each)using a random number table method:CD+solvent group(CD+C group)and CD+TRPA1 agonist allyl isothiocyanate(AITC)group(CD+A group).The mice in group HFD were divided into 2 groups(n=6 each)using a random number table method:HFD+solvent group(HFD+C group)and HFD+AITC group(HFD+A group).At 13th week,AITC 25 mg·kg^(-1)·d^(-1)was intragastrically administered based on the original diet in CD+A group and HFD+A group,and the equal volume of solvent was given in CD+C and HFD+C groups.At 14th week,myocardial I/R injury was established by occlusion of the left coronary artery for 30 min followed by 120-min reperfusion.The body weight,epididymal fat,mesenteric fat and perirenal fat were recorded,and the levels of LDH,triglyceride and cholesterol in serum were determined.The percentage of myocardial infarct size was calculated.The expression of TRPA1,Bax and Bcl-2 was detected by Western blot,and the ratio of Bax/Bcl-2 was calculated.Results Compared with CD+C group,the expression of TRPA1 in myocardial tissues was significantly up-regulated(P<0.05),and no significant differences were found in the other parameters in CD+A group(P>0.05),and the concentrations of serum triglyceride and cholesterol,body weight,weight of epididymal fat,mesenteric fat,perirenal fat and percentage of myocardial infarct size were significantly increased,the expression of TRPA1 in myocardial tissues was down-regulated,and the concentration of LDH and rat

关 键 词：肥胖症 心肌再灌注损伤 瞬时受体电位通道 

分 类 号：R614[医药卫生—麻醉学]