丹酚酸B对支原体肺炎幼鼠肺组织的保护作用及可能机制  

Protective effect and its possible mechanism of salvianolic acid B on lung tissue of young mice with mycoplasma pneumonia

作　　者：肖韵 王建刚 王莹巍 王娟[2] XIAO Yun;WANG Jian-gang;WANG Ying-wei;WANG Juan(Department of Paediatrics,Yingkou Central Hospital,Yingkou 115003;Department of Pediatric Respiratory Medicine,Shengjing Hospital Affiliated to China Medical University,Shenyang 110004,China)

机构地区：[1]营口市中心医院儿科,辽宁营口115003 [2]中国医科大学附属盛京医院小儿呼吸内科,辽宁沈阳110004

出　　处：《解剖科学进展》2024年第2期128-130,共3页Progress of Anatomical Sciences

摘　　要：目的丹酚酸B对支原体肺炎幼鼠肺组织的保护作用及可能机制。方法将60只SPF级BALB/c幼鼠随机分为对照组(Control组)、支原体肺炎组(MP组)和丹酚酸B组(Sal B组),每组20只。HE染色方法观察各组幼鼠肺组织形态学变化;TUNEL方法检测各组幼鼠肺组织细胞的凋亡;ELISA方法检测各组幼鼠血清白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)含量;Western blot方法检测各组幼鼠肺组织低氧诱导因子-1(hypoxia-inducible factor-1,HIF-1)与活化的半胱氨酸天冬氨酸蛋白酶3(cleaved caspase-3)表达。结果丹酚酸B能够减轻MP幼鼠肺组织炎性程度,减少MP幼鼠肺组织细胞的凋亡水平,降低MP幼鼠血清炎症因子IL-1β、TNF-α和IL-6的含量,降低MP幼鼠肺组织HIF-1与cleaved caspase-3蛋白的表达。结论丹酚酸B能通过抑制HIF-1与cleaved caspase-3的蛋白表达减轻支原体肺炎幼鼠肺组织损伤。Objective To explore the protective effect and its possible mechanism of salvianolic acid B on lung tissue of young mice with mycoplasma pneumonia.Methods Sixty SPF grade BALB/c young mice were randomly divided into control group(Control group),mycoplasma pneumonia group(MP group)and salvianolic acid B group(Sal B group),with 20 mice in each group.The morphological changes of lung tissue were observed by HE staining.The apoptosis of lung tissue cells of young mice in each group was observed by TUNEL method.The levels of interleukin-1β,interleukin-6 and tumor necrosis factor-α(TNF-α)in serum of young mice were detected by ELISA.The expressions of hypoxia-inducible Fact-1(HIF-1)and cleaved caspase-3 in lung tissues of young mice in each group were detected by Western blot.Results Salvianolic acid B could reduce the inflammation in lung tissue of young mice with mycoplasma pneumonia,reduce the apoptosis of lung tissue cells,reduce the levels of IL-1β,TNF-αand IL-6 in serum,and reduce the expressions of HIF-1 and cleaved caspase-3 in lung tissue of young mice with mycoplasma pneumonia.Conclusion Salvianolic acid B can reduce lung tissue injury in young mice with mycoplasma pneumonia by inhibiting the expressions of HIF-1 and cleaved caspase-3.

关 键 词：丹酚酸B 支原体肺炎 低氧诱导因子-1 cleaved caspase-3 

分 类 号：R563.1[医药卫生—呼吸系统]