Salusin-β调控NF-κB通路促进2型糖尿病性干眼病的炎症反应  

Salusin-βpromotes the inflammatory response in type 2 diabetic dry eye by regulating the NF-κB pathway

作　　者：王豪[1] 欧升聪 张学东[3] WANG Hao;OU Sheng-cong;ZHANG Xue-dong(Department of Ophthalmology,Fuling Hospital Affiliated to Chongqing University,Chongqing 408030;Department of Ophthalmology,Changshou District Hospital of Traditional Chinese Medicine,Chongqing 401220;Department of Ophthalmology,First Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)

机构地区：[1]重庆大学附属涪陵医院眼科,重庆401220 [2]重庆市长寿区中医院眼科,重庆401220 [3]重庆医科大学附属第一医院眼科,重庆401220

出　　处：《解剖科学进展》2024年第2期146-148,共3页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81870673)

摘　　要：目的探究Salusin-β与NF-κB通路在2型糖尿病性干眼病炎症反应中的作用。方法将30只健康雄性Wistar大鼠随机分为空白对照组、阴性对照组、Salusin-β低表达组,每组10只。HE染色观察大鼠眼结膜的病理结构变化;酶联免疫吸附法测定泪液炎症因子的水平;蛋白免疫印迹实验分析大鼠结膜组织Salusin-β和NF-κB p65蛋白的表达。结果与阴性对照组比较,低表达组的大鼠眼表病变情况显著减轻、泪液分泌增加、角膜增厚、炎症细胞浸润和基质水肿的情况均得到改善、泪液中炎症因子显著降低、眼结膜Salusin-β和NF-κB p-p65蛋白表达水平显著降低。结论Salusin-β可能通过调控NF-κB通路促进2型糖尿病性干眼病的炎症反应。Objective To investigate the role of Salusin-βand NF-κB pathway in the inflammation of type 2 diabetic dry eye.Methods Thirty healthy male Wistar rats were randomly divided into blank control group,negative control group and Salusin-βlow expression group,10 in each group.The pathological changes of rat conjunctiva were observed by HE staining.The levels of inflammatory factors in tears were measured by enzyme-linked immunosorbent assay.The expressions of Salusin-βand NF-κB p65 protein in rat conjunctiva was analyzed by Western blot.Results Compared with the negative control group,the ocular surface lesions of the rats in the Salusin-βlow expression group were significantly alleviated,the tear secretion was increased,the corneal thickening,inflammatory cell infiltration and matrix edema were improved,the inflammatory factors in tears were significantly reduced,and the levels of Salusin-βand NF-κB p-p65 in conjunctiva were significantly decreased.Conclusion Salusin-βmight promote inflammation in type 2 diabetic dry eye disease by regulating the NF-κB pathway.

关 键 词：Salusin-β NF-ΚB 2型糖尿病 干眼 

分 类 号：R587.2[医药卫生—内分泌]