Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex  

作　　者：Yang Qin Jiangtian Li Xuehao Zhang Kaixuan Wan Heao Zhang Feiyang Huang Limei Wang Hongxun Wang Longjie Li Xianjin Xiao 

机构地区：[1]Institute of Reproductive Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [2]Department of Pancreatic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China [3]Wuhan Polytechnic University,Wuhan 430023,China

出　　处：《Chinese Chemical Letters》2024年第5期330-337,共8页中国化学快报（英文版）

基　　金：financially supported by the National Key Research and Development Program of China(No.2021YFC2701402);the Open Research Fund of State Key Laboratory of Bioelectronics,Southeast University(No.Sklb2021-k06);the Open Foundation of NHC Key Laboratory of Birth Defect for Research and Prevention(Hunan Provincial Maternal and Child Health Care Hospital)(No.KF2020007);the Open Foundation of Translational Medicine National Science and Technology Infrastructure(Shanghai)(No.TMSK-2021-141)。

摘　　要：Strand displacement reaction is a crucial component in the assembly of diverse DNA-based nanodevices,with the toehold-mediated strand displacement reaction representing the prevailing strategy.However,the single-stranded Watson-Crick sticky region that serves as the trigger for strand displacement can also cause leakage reactions by introducing crosstalk in complex DNA circuits.Here,we proposed the toeless and reversible DNA strand displacement reaction based on the Hoogsteen-bond triplex,which is compatible with most of the existing DNA circuits.We demonstrated that our proposed reaction can occur at pH 5 and can be reversed at pH 9.We also observed an approximately linear relationship between the degree of reaction and pH within the range of pH 5-6,providing the potential for precise regulation of the reaction.Meanwhile,by altering the sequence orientation,we have demonstrated that our proposed reaction can be initiated or regulated through the same toeless mechanism without the requirement for protonation in low pH conditions.Based on the proposed reaction principle,we further constructed a variety of DNA nanodevices,including two types of DNA logic gates that rely on pH 5/pH 9 changes for initiating and reversing:the AND gate and the OR gate.We also successfully constructed a DNA Walker based on our proposed reaction modes,which can move along a given track after the introduction of a programmable DNA sequence and complete a cycle after 4 steps.Our findings suggest that this innovative approach will have broad utility in the development of DNA circuits,molecular sensors,and other complex biological systems.

关 键 词：DNA strand displacement Toeless Triplex DNA strands REVERSIBLE DNA circuit DNA walker 

分 类 号：Q75[生物学—分子生物学]