冠菌素诱导番茄对青枯病原菌(Ralstonia solanacearum)抗性的转录组分析  

Transcriptome analysis of coronatine-induced resistance to Ralstonia solanacearum in tomato plants

作　　者：李娜[1] 邹丽芳[2] 辛屹东 孙胜[1] 耿雪青 LI Na;ZOU Lifang;XIN Yidong;SUN Sheng;GENG Xueqing(College of Horticulture,Shanxi Agricultural University,Taigu 030801,China;School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]山西农业大学园艺学院,太谷030801 [2]上海交通大学农业与生物学院,上海200240

出　　处：《植物病理学报》2024年第2期343-354,共12页Acta Phytopathologica Sinica

基　　金：上海市科技兴农技术创新项目(2021-02-08-00-12-F00799);山西省重点研发项目(202102140601015);山西省现代农业(蔬菜)产业体系建设专项资金;晋中国家农高区番茄智慧标准化技术研究教授博士工作站启动专项(JZNGQBSGZZ004)。

摘　　要：青枯雷尔氏菌(Ralstonia solanacearum)引起的青枯病严重威胁着番茄的产量和品质。冠菌素(Coronatine,COR)是由丁香假单胞菌(Pseudomonas syringae)产生的化合物,结构类似于茉莉酸异亮氨酸(JA-Ile)。本研究通过观察COR处理番茄后接种R.solanacearum的症状表现,发现COR处理的番茄植株比未处理的对照组青枯病发生症状轻。为进一步探究COR如何影响番茄对青枯病的抗性,对COR处理后接种R.solanacearum 24 h的番茄样品进行转录组测序分析,结果发现,COR处理共诱导了2122个差异表达基因,包括998个上调表达基因和1124个下调表达基因。通过对差异表达基因进行GO和KEGG富集,发现COR主要影响植物-病原菌互作通路及植物激素信号转导途径相关基因的表达。此外,COR还诱导JA合成通路相关基因的上调表达,以及抑制光合作用相关基因的表达。研究结果为深入揭示COR在植物-青枯病原菌互作过程中的作用奠定了理论基础。The bacterial wilt caused by Ralstonia solanacearum has brought a serious threat to tomato production.Coronatine(COR),structurally similar to JA-Ile(JA-isoleucine),is a compound produced by Pseudomonas syringae.In this study,we observed that pre-treatment of tomato seedlings with COR alleviated the symptoms caused by R.solanacearum.To investigate how COR affects tomato resistance to R.solanacearum,transcriptome sequencing of tomato seedlings inoculated with the pathogen 24 h after COR treatment was carried out.Analysis of RNA-seq data showed that COR treatment induced a total of 2122 differentially expressed genes(DEGs),including 998 up-regulated genes and 1124 down-regulated genes.DEGs annotation and pathway enrichment were conducted using GO database and KEGG database,and the results showed that COR affected the expression of genes related to plant-pathogen interaction pathway and plant hormone signaling pathways.Meanwhile,COR induced the up-regulation of genes involved in the jasmonic acid synthesis pathway and inhibited the expression of photosynthesis-related genes.Our results provide a theoretical basis for revealing the role of COR in plant-microbe interaction.

关 键 词：冠菌素 青枯病原菌 番茄 转录组测序 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]