生防链霉菌菌株S16鉴定和防蔬菜根肿病的潜力评价  

Identification of Streptomyces strain S16 and evaluating its biocontrol potential against Plasmodiophora brassicae

作　　者：贺赛雅 袁加升 周星海 刘学瑞 魏兰芳[1,2] 姬广海[1] HE Saiya;YUAN Jiasheng;ZHOU Xinghai;LIU Xuerui;WEI Lanfang;JI Guanghai(State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan Agricultural University,Kunming 650201,Yunnan,China;Agricultural Foundation Experiment Teaching Center,Yunnan Agricultural University,Kunming 650201,Yunnan,China)

机构地区：[1]云南农业大学省部共建云南生物资源保护与利用国家重点实验室,云南昆明650201 [2]云南农业大学农科基础实验教学中心,云南昆明650201

出　　处：《植物病理学报》2024年第2期410-418,共9页Acta Phytopathologica Sinica

基　　金：国家自然科学基金委员会-地区基金项(32260701)。

摘　　要：【目的】为了寻找新的生防资源应对日益严重十字花科蔬菜根肿病。【方法】本实验从根肿病重病田仅存的几株健康白菜的根际土壤中,通过培养组学分离到485株细菌,以辣椒疫霉Phytophthora capsici为指示菌,利用平板对峙和温室盆栽试验筛选对白菜根肿病的生防菌株,并通过形态学特征、16S rDNA序列对其进行鉴定,利用热处理、酸碱处理、紫外线处理和蛋白酶K处理对生防菌的稳定性进行测定,同时利用平板对峙的方法测定了该菌的抑菌谱。【结果】通过对峙培养筛选获得46株有抑菌效果的生防菌株,并利用盆栽实验进行复筛,获得1株对白菜根肿病具有较好防治效果的菌株,命名为S16。该菌株S16对60℃以上的高温比较敏感;对紫外线照射不敏感,有较强的UV稳定性;pH在4~8处理后稳定性较好,且酶处理对菌株产生的抗菌物质几乎没影响。结合形态学特征和系统发育分析结果将该菌株鉴定为灰色橙黄链霉菌Streptomyces griseoaurantiacus。S16对辣椒疫霉Ph.capsici等8种病原真菌具有抑制效果,抑菌谱较广,对白菜根肿病的盆栽防效达到68.74%。【结论】表明灰色橙黄链霉菌S16对十字花科根肿病具有显著的防效,可作为生防菌进一步开发利用。[Objective]In order to find new biocontrol resources to address the increasing severity of cruciferous vegetable clubroot disease.[Method]In our study,485 strains of bacteria were isolated from the rhizosphere soil of only a few healthy Chinese cabbage plants in the field of serious clubroot disease.Using Phytophthora capsici as an indicator fungus,we screened the biocontrol strains of cabbage clubroot disease by plate standoff and greenhouse pot experiment.Morphological characteristics and 16S rDNA sequence were used to identify the biocontrol bacteria.The stability of biocontrol bacteria was measured by heat treatment,acid-base treatment,ultraviolet treatment and protease K treatment.At the same time,the plate confrontation method was used to determine the antibacterial spectrum of the bacteria.[Result]46 biocontrol strains with antibacterial effects were obtained through standoff culture screening,and one strain with good control effect on Chinese cabbage clubroot disease was obtained through pot experiment re-screening.The strain S16 is relatively sensitive to high temperatures above 60℃,not sensitive to ultraviolet radiation and has strong UV stability.The stability is good after pH treatment at 4~8,and enzyme treatment has almost no effect on the antibacterial substances produced by the strain.Based on morphological characteristics and phylogenetic analysis,the strain was identified as Streptomyces griseoaurantiacus.S16 has inhibitory effects on Phytophthora capsici and other 7 pathogenic fungi,with a wide antibacterial spectrum,and a pot culture control effect of 68.74%on Chinese cabbage root swelling disease.[Conclusion]This indicates that S.griseoaurantiacus strain S16 has significant control effects on the clubroot disease of the cruciferous vegetable,and can be further developed into a biocontrol bacterium.

关 键 词：十字花科蔬菜 根肿病 灰色橙黄链霉菌 生物防治 

分 类 号：S432.1[农业科学—植物病理学]