3种黄矮病毒多重RT-PCR检测方法的建立及应用  

Establishment and application of multiple RT-PCR for detection of three yellow dwarf viruses

作　　者：王贺 姜兴林 韩晓玉 徐永伟 彭红 王怡凡 袁虹霞[1] 李洪连[1,3,4] 杨雪 施艳 WANG He;JIANG Xinglin;HAN Xiaoyu;XU Yongwei;PENG Hong;WANG Yifan;YUAN Hongxia;LI Honglian;YANG Xue;SHI Yan(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China;Crop Science Postdoctoral Programme of Henan Agricultural University,Zhengzhou 450002,China;Collaborative Innovation Centre of Henan Grain crops,Zhengzhou 450002,China;State Key Laboratory of Wheat and Maize Crop Science,Zhengzhou 450002,China;Henan Plant Protection and Plant Quarantine Station,Zhengzhou 450002,China)

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]河南农业大学作物学博士后流动站,郑州450002 [3]河南省粮食作物协同创新中心,郑州450002 [4]小麦玉米作物学国家重点实验室,郑州450002 [5]河南省植物保护检疫站,郑州450002

出　　处：《植物病理学报》2024年第2期457-461,共5页Acta Phytopathologica Sinica

基　　金：河南省重点研发专项(231111111100);河南省玉米产业技术体系植保岗位科学家科研专项(HARS-22-02-C3);河南省青年人才托举工程项目(2022HYTP037);河南农业大学科技创新项目(KJCX2021A13)。

摘　　要：0 引言.小麦黄矮病是我国小麦生产上重要的病毒病,受害小麦感病后植物矮化叶片黄化,一般减产10%~20%左右,严重的可达到50%以上,个别地块甚至可造成绝产。目前国际上已报道的作物黄矮病毒有十多种[1],我国引起小麦黄矮病的主要病原是大麦黄矮病毒(barley yellow dwarf virus, BYDV)PAV和GAV株系[2],其在全国小麦种植区广泛分布。Wheat yellow dwarf disease causes typical disease symptoms such as yellowing,stunting,reduced seed size and yield,leading to severe economic loss in wheat production.Two barley yellow dwarf virus(BYDV)lines,BYDV-PAV and BYDV-GAV,are main viruses causing yellow dwarf in wheat.Maize yellow mosaic virus(MaYMV)was recently reported to cause yellow dwarf in wheat.In this study,diseased leaf samples showing yellowing and stunting on wheat were collected in various regions in Henan Province in 2022.The optimal reaction condition for multiple RT-PCR detection of BYDV-PAV,BYDV-GAV and MaYMV was established with the following condition:final primer concentration at 0.1μmol·L-1,annealing temperature at 55℃,annealing and extension time of 90 s and 120 s,respectively.We further used the established multiple RT-PCR system to detect 19 field samples.It showed that 11 out of 19 samples were positive for both BYDV-PAV and MaYMV,indicating that the mixed infection of BYDV-PAV and MaYMV was common in the field.Our study laid the foundation for the field surveillance and control of wheat yellow dwarf disease in Henan Province.

关 键 词：小麦黄矮病 我国小麦生产 大麦黄矮病毒 叶片黄化 主要病原 yellow BYDV 

分 类 号：S432.44[农业科学—植物病理学]