衣壳蛋白潜在乙酰化位点在马铃薯Y病毒侵染过程中的作用  

A role for coat protein potential acetylation sites in potato virus Y infection

作　　者：姬丽云 魏新钰 周令希 王凤龙[2] 田延平[1] 李向东[1] JI Liyun;WEI Xinyu;ZHOU Lingxi;WANG Fenglong;TIAN Yanping;LI Xiangdong(Department of Plant Pathology,College of Plant Protection,Shandong Agricultural University,Tai'an 271018,China;Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 276101,China)

机构地区：[1]山东农业大学植物保护学院植物病理学系,泰安271018 [2]中国农业科学院烟草研究所,青岛276101

出　　处：《植物病理学报》2024年第2期462-468,共7页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31720103912、32072387、31871933);山东省烟草公司(KN275)。

摘　　要：0 引言.马铃薯Y病毒(potato virus Y,PVY)是马铃薯Y病毒属(Potyvirus)的重要成员,可侵染170多种植物,其中茄科、藜科和豆科植物受害最为严重。1931年SMITH首次在马铃薯中发现PVY[1]。通过乙酰化组学在衣壳蛋白(coat protein, CP)中发现多个潜在乙酰化位点,但它们在PVY侵染中的作用不清楚。本研究通过突变解析了CP中潜在乙酰化位点在PVY侵染珊西烟的致病力变化.Potato virus Y(PVY)belongs to the genus Potyvirus.The multifunctional protein coat protein(CP)of PVY is involved in the virus genome replication,aphid transmission,cell-to-cell and long-distance movement,symptom formation,etc.Our previous results have revealed that multiple sites within CP are potentially acetylated.However,the effect of acetylation on those potential sites of CP on PVY infection is still unclear.In this study,we mutated the identified sites,K12,K13,K16,K28 and K32 located at the N terminal,K177,K221 and K226 located at the central domain and K265 located at the C-terminal,to acetylation mimicking residue glutamine and non-acetylation residue arginine,respectively.We analyzed the infectivity of 18 PVY mutants on Nicotiana tabacum cv Xanthi plants.It showed that most of the mutants containing a single mutation have no apparent effect on the pathogenicity of PVY on N.tabacum cv Xanthi.Non-acetylation mutation at sites K16 and K265 abolished the ability of PVY to induce vein necrosis.Both non-acetylation and acetylation at site K221 reduced PVY replication,and these modifications also abolished the ability of PVY mutants to move between cells.This is the first report to reveal the effect of potential acetylation in CP on PVY infection.These results will enhance our understanding of the role of post-translational modification during PVY infection.

关 键 词：马铃薯Y病毒 侵染过程 衣壳蛋白 致病力 PVY 豆科植物 乙酰化修饰 

分 类 号：S432.41[农业科学—植物病理学]