河南省地黄病毒病病原鉴定及主要病毒的分子变异分析  

Identification of the pathogens of Rehmannia glutinosa viral disease and molecular variation analysis of the major viruses

作　　者：秦艳红[1] 文艺[1] 高素霞[1] 张德胜[1] 刘玉霞[1] 刘永康 李绍建[1] 赵正伟 王凤丽 王飞[1] 鲁传涛[1] QIN Yanhong;WEN Yi;GAO Suxia;ZHANG Desheng;LIU Yuxia;LIU Yongkang;LI Shaojian;ZHAO Zhengwei;WANG Fengli;WANG Fei;LU Chuantao(Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Wenxian Agriculture Science Research Institute,Wenxian 475004,China)

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002 [2]温县农业科学研究所,温县454850

出　　处：《植物病理学报》2024年第2期469-475,共7页Acta Phytopathologica Sinica

基　　金：国家中药材产业技术体系(CARS-B21);河南省中药材产业技术体系(HARS-22-11-Z1);河南省农业科学院基本科研业务费项目(2023ZC051)。

摘　　要：0 引言.地黄(Rehmannia glutinosa L.)为玄参科地黄属多年生草本植物,以干燥块根入药,是我国重要的中药材之一,具有清热凉血、养阴生津、降血糖、保护肝脏等功效[1]。地黄病毒病是地黄上的严重病害之一,地黄感染病毒后,产量可降低60%以上,有效成分降低30%~50%[2]。A total of 60 leaf samples with mosaic and chlorotic symptoms were collected from the main planting areas of Rehmannia glutinosa in Henan province.High throughput sequencing and RT-PCR assays were used to detect viruses in the samples.The results showed that five virus species and one viroid were detected from the R.glutinosa samples of Henan province,including youcai mosaic virus(YoMV),rehmannia mosaic virus(ReMV),broad bean wilt virus 2(BBWV2),tobacco mild green mosaic virus(TMGMV),cucurbit chlorotic yellows virus(CCYV)and columnea latent viroid(CLVd).This is the first report of CLVd infection on R.glutinosa.All 60 samples were positive and the detection rates of YoMV,ReMV,BBWV2,TMGMV,CCYV and CLVd were 100%,93.3%,85.0%,78.3%,36.7%and 8.3%,respectively.All of these samples(100%)were found to be infected by two or more of the above viruses.The detection rate of co-infection by 3-6 kinds of viruses were 96.7%,and co-infection by 4 kinds of viruses were the major mixed type(40.0%).Combined infection by YoMV+ReMV+TMGMV+BBWV2 was the most common mixed type.Molecular variation analysis showed that CLVd was the most highly conserved,followed by YoMV and ReMV,but BBWV2 showed more variation.Phylogenetic tree analyses indicated that the isolates of YoMV and ReMV had a host specificity correlation,but no obvious host specificity and geographic differentiation correlation were found among the isolates of BBWV2 and CLVd.

关 键 词：烟草花叶病毒 黄瓜花叶病毒 番茄花叶病毒 油菜花叶病毒 清热凉血 养阴生津 黄病毒病 病原鉴定 

分 类 号：S432.41[农业科学—植物病理学]