利用分子传播网络分析昆明市HIV-1 CRF01_AE毒株流行特点  

Using molecular transmission networks to analyze the epidemiological characteristics of HIV-1 CRF01_AE in Kunming

作　　者：程鹏 刘家法 王佳丽 杨翠先 何保翠 张米 李健健 董兴齐 CHENG Peng;LIU Jiafa;WANG Jiali;YANG Cuixian;HE Baocui;ZHANG Mi;LI Jianjian;DONG Xingqi(Yunnan Infectious Disease Hospital,Department of Laboratory,Yunnan AIDS Care Center,Kunming 650301,China;Department of Epidemiology,School of Public Health,Kunming Medical University,Kunming 650000,China;Office of the Chief Expert,Yunnan Infectious Disease Hospital,Yunnan AIDS Care Center,Kunming 650301,China)

机构地区：[1]云南省传染病医院,云南省艾滋病关爱中心检验科,昆明650301 [2]昆明医科大学公共卫生学院流行病学教研室,昆明650000 [3]云南省传染病医院,云南省艾滋病关爱中心首席专家办公室,昆明650301

出　　处：《中华疾病控制杂志》2024年第5期574-580,585,共8页Chinese Journal of Disease Control & Prevention

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项资金(202201AY070001-208,202101AY070001-223);云南省艾滋病病毒学及临床诊疗技术创新研究中心项目(202102AA310005);云南省教育厅创新团队项目(CXTD202111)。

摘　　要：目的 通过构建昆明市HIV-1 CRF01_AE毒株的分子传播网络,分析其流行特征并观察动态流行趋势,从而为当地制定疫情防控干预措施提供科学依据。方法 选择2015―2021年云南省传染病医院接受治疗的253例昆明市HIV-1 CRF01_AE新发感染患者作为研究对象。运用反转录巢式聚合酶链式反应成功扩增其基因序列,序列比对后导入HyPhy 2.2.4软件进行成对基因距离的计算。运用GraphPad-Prism 8.0软件确定最佳基因距离阈值,使用Cytoscope 3.7.2软件实现网络可视化。运用Network-Analyzer和MCODE(Molecular Complex Detection)工具进行网络特征分析。结果 在0.018最佳基因距离阈值下,253个研究对象中有118个(46.64%)进入网络分析,共形成了38个分子簇,其组成大小从2到17个节点不等。网络集群主要以异性传播(51.78%)和同性传播(37.94%)为主,年龄段主要分布于20~40岁(77.47%)。网络所有节点的总链接数为226,单个节点最高链接数为10。MCODE确定了3种重要的分子簇,3个分子簇分别定义为B、C、D型,B型由17个节点和19链接数组成,为同性传播集群呈低增长状态。C和D型均由5个节点和10链接数组成,为异性传播集群且呈静止状态。结论 昆明市HIV-1 CRF01_AE毒株分子传播网络中的分子簇具有一定特殊性和聚集性。同性传播和异性传播人群已经成为昆明市HIV-1 CRF01_AE毒株感染的两大风险群体,同时两个群体还有发生交叉传播的现象。传播网络中有一个同性传播组成的集群规模较大,其传播风险和活跃度较高,需加强对其监测,并制定针对性的干预措施对集群中的“核心人群”进行防控。Objective To construct the molecular transmission network of the HIV-1 CRF01_AE strain in Kunming,to analyze its epidemiological characteristics,to observe its dynamic epidemiological trends,and to provide a scientific basis for the development of local epidemic prevention and control interventions.Methods A total of 253 newly infected patients with HIV-1 CRF01_AE in Kunming who received treatment at Yunnan Infectious Disease Hospital from 2015 to 2021 were selected as study subjects.Their gene sequences were successfully amplified using RT-nested PCR(reverse transcription nested polymerase chain reaction),and the sequences were compared and imported into HyPhy 2.2.4 software for the calculation of paired gene distances.GraphPad-Prism 8.0 software was used to determine the optimal gene distance threshold,and Cytoscope 3.7.2 software was used for network visualization.Network characterization was performed using the Network Analyzer and MCODE(Molecular Complex Detection)tools.Results At an optimal genetic distance threshold of 0.018,118 of 253 study subjects(46.64%)entered the network analysis,forming a total of 38 molecular clusters with composition sizes ranging from 2 to 17 nodes.The network clusters were mainly heterosexual(51.78%)and homosexual(37.94%),and the age group was mainly distributed between 20 and 40 years old(77.47%).The total number of links for all nodes in the network was 226;the maximum number of links for a single node was 10.MCODE identified three important molecular clusters,types B,C and D.Type B consisted of 17 nodes and 19 link counts and was a homosexually propagating cluster in a low-growth state.Types C and D both consisted of 5 nodes and 10 link counts,were heterosexually propagating clusters,and were stationary.Conclusions The molecular clusters in the molecular transmission network of the HIV-1 CRF01_AE strain in Kunming have certain specificity and aggregation.Homosexual and heterosexual transmission populations have become the two major risk groups for HIV-1 CRF01_AE strain infect

关 键 词：HIV-1 CRF01_AE 分子传播网络 成对基因距离 分子簇 

分 类 号：R512.91[医药卫生—内科学] R181.3[医药卫生—临床医学]