AMF和DSE对紫花苜蓿生长发育及叶片代谢组分的影响  

Effects of AMF and DSE on the growth and development and metabolic fractions of leaves of Medicago sativa

作　　者：毕银丽 宋雅宁[1,2] 白雪蕊 王淑惠 BI Yinli;SONG Yaning;BAI Xuerui;WANG Shuhui(Institute of Ecological Environment Restoration in Mine Areas of West China,Xi'an University of Science and Technology,Xi'an 710054,Shaanxi,China;College of Geology and Environment,Xi'an University of Science and Technology,Xi'an 710054,Shaanxi,China;State Key Laboratory for Coal Resources and Safe Mining,China University of Mining and Technology,Beijing 100083,China)

机构地区：[1]西安科技大学西部矿山生态环境修复研究院,陕西西安710054 [2]西安科技大学地质与环境学院,陕西西安710054 [3]中国矿业大学(北京)煤炭资源与安全开采国家重点实验室,北京100083

出　　处：《菌物学报》2024年第5期80-94,共15页Mycosystema

基　　金：国家重点研发计划项目(2022YFF1303300);黄河中心专项(2022-YRUC-01-0304)。

摘　　要：丛枝菌根真菌(arbuscularmycorrhizalfungi,AMF)和深色有隔内生真菌(darkseptate endophytes,DSE)能与植物形成良好的共生关系,并促进植物的生长发育。然而,当前AMF和DSE促进植物生长发育的调控机制在很大程度上仍不清楚。为探究AMF和DSE对紫花苜蓿的促生作用及叶片代谢组分的影响,通过温室盆栽模拟实验,设计CK、AMF、DSE、AMF+DSE4种接种处理,测定在土壤灭菌条件下不同处理对紫花苜蓿幼苗根系侵染率、生理生长、营养状况等指标。同时,建立基于超高效液相色谱-四极杆飞行时间质谱技术(UPLC-Q-TOF-MS)的非靶向代谢组学方法,分析不同处理紫花苜蓿叶片代谢产物的差异。结果表明:AMF和DSE促进紫花苜蓿根系发育(根长、根体积、根尖数、根表面积和根投影面积)、增加紫花苜蓿生物量、叶绿素和营养元素含量。紫花苜蓿叶片代谢组学分析共鉴定出91种代谢产物,其中脂质和类脂分子的含量占比最大,且接菌增加了代谢产物中脂质和类脂分子的比例,通路分析表明,接菌对甘油磷脂代谢、半乳糖代谢和卟啉叶绿素代谢影响较大。与其他处理相比,影响代谢途径的关键代谢产物磷脂酰乙醇胺、甘油、棕榈酸、β-胡萝卜素在AMF+DSE联合接种中含量最高。本研究结果为AMF和DSE对紫花苜蓿的生长和代谢机制的影响提供理论依据。Arbuscular mycorrhizal fungi(AMF)and dark septate endophytes(DSE)can form symbiotic relationships with plants and promote their growth and development.However,the regulatory mechanisms by which AMF and DSE promote plant growth and development are largely unknown.In order to study the effects of AMF and DSE on the growth-promotion and metabolic components of Medicago sativa leaves,a greenhouse pot simulation experiment was designed and CK,AMF,DSE and AMF+DSE inoculation treatments were set up.The effects of different treatment on the root colonization,physiological growth,and nutrient uptake of M.sativa were studied under soil sterilization conditions.A non-targeted metabolomics method for identifying the metabolites of M.sativa leaves was established by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).The results show that the addition of AMF and DSE promoted M.sativa root development(root length,root volume,root tip number,root surface area and root projected area)and increased M.sativa biomass,chlorophyll and nutrient content.A total of 91 metabolites was identified by metabolomics analysis of leaves,belonging to different chemical groups of which the content of lipids and lipid-like molecules accounted for the largest proportion.The proportion of lipids and lipid-like molecules in the leaves increased as a result of AMF and DSE treatment.Pathway analysis showed that AMF and DSE relatively influence glycerophospholipid metabolism,galactose metabolism,and porphyrin and chlorophyll metabolism.The content of key metabolites affecting the metabolic pathways,phosphatidylethanolamines,CDP-glycerol,palmitic acid,and beta-carotene,were the highest under the condition of AMF+DSE co-inoculation as compared to that under other treatments.The results prove that AMF and DSE evidently stimulate the growth and metabolism of M.sativa.

关 键 词：丛枝菌根真菌 深色有隔内生真菌 超高效液相色谱 四极杆飞行时间质谱 植物代谢组学 

分 类 号：S541.9[农业科学—作物学]