薰衣草LibHLH75基因的克隆、表达及功能初步分析  

Cloning,Expression and Preliminary Functional Analysis of LibHLH75 Gene from Lavender(Lavandula×intermedia)

作　　者：买尔孜亚古丽·阿不来克木 张佳俊 严中建 廖燕 石梅兰 李雪龙 王爱凡 苏秀娟[1] Maierziyaguli·ABULAIKEMU;ZHANG Jia-Jun;YAN Zhong-Jian;LIAO Yan;SHI Mei-Lan;LI Xue-Long;WANG Ai-Fan;SU Xiu-Juan(College of Agriculture/Xinjiang Key Lab of Crop Improvement&Germplasm Enhancement/Lavender Research Institute,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学农学院/新疆作物遗传改良与种质创新重点实验室/薰衣草研究所,乌鲁木齐830052

出　　处：《农业生物技术学报》2024年第6期1307-1315,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金地区科学基金(31960452);新疆农业大学研究生创新项目(XJAUGRI2022037);大学生创新项目(DXSCX2021022);自治区“天山英才”培养计划青年拔尖人才项目(2023TSYCCX0017)。

摘　　要：薰衣草(Lavandula)是重要的芳香作物,其精油品质的差异主要由芳樟醇和乙酸芳樟酯等萜烯类化合物的含量不同而引起。对薰衣草中可能参与调控精油主要成分生物合成的相关基因进行研究,可为培育薰衣草优异种质提供基因资源。碱性螺旋-环-螺旋(basic Helix-Loop-Helix,bHLH)转录因子家族在植物的生长发育和次生代谢等方面发挥着重要的作用,本研究以‘杂花’薰衣草(Lavandula×intermedia)为材料,克隆了薰衣草LibHLH75基因(GenBank No.OR019688),其开放阅读框为831 bp,编码276个氨基酸,其蛋白与一串红(Salvia splendens)bHLH75-like蛋白和芡欧鼠尾草(S.hispanica)b HLH75蛋白的亲缘关系最近。qRT-PCR分析表明,LibHLH75基因在薰衣草不同组织及花冠的不同开花期均有表达,其在花萼和花蕾期的表达量最高。亚细胞定位和转录激活活性鉴定结果表明,LibHLH75基因编码的蛋白定位在细胞核内,且具有转录激活活性。本研究初步分析了LibHLH75基因的功能,为进一步揭示b HLH转录因子在薰衣草萜类化合物生物合成过程中的分子调控机制提供了理论依据。Lavender(Lavandula)is an important aromatic crop,and the differences in the quality of its essential oil are mainly caused by variations in the content of terpenoids,such as linalool and linalyl acetate.Studying the relevant genes that may be involved in regulating the biosynthesis of essential oils in lavender can provide genetic resources for breeding excellent lavender germplasm.The basic Helix-Loop-Helix(bHLH)transcription factor family plays a crucial role in plant growth,development,and secondary metabolism.In this study,the lavender LibHLH75 gene(GenBank No.OR019688)was cloned from the'Zahua'of lavender(Lavandula×intermedia),with an open reading frame of 831 bp,encoding 276 amino acids,which was closest to the bHLH75-like protein from Salvia splendens and bHLH75 protein from S.hispanica.qRT-PCR analysis showed that the LibHLH75 gene was expressed in different tissues of lavender and at different flowering stages of the corolla,with the highest expression level at the calyx and bud stages.The results of subcellular localization and transcriptional activation activity identification indicated that LibHLH75 encoded protein was localized in the nucleus,and had transcriptional activation activity.This study preliminary analyzed the function of LibHLH75,which provides a theoretical basis for further revealing the molecular regulatory mechanism of bHLH transcription factors in the biosynthesis of lavender terpenoids.

关 键 词：‘杂花’薰衣草 LibHLH75转录因子 基因克隆 表达模式 转录激活 

分 类 号：S573.9[农业科学—作物学]