Hippo信号通路下游关键因子YAP和TEAD-1在牦牛胎盘发育中的表达与定位  

Expression and Localization of Key Factors YAP and TEAD-1 in the Downstream of Hippo Signaling Pathway During Placental Development in Yak(Bos grunniens)

作　　者：钱文洁 杜培岩 李一娟 周应聪 姚颖 李柳杨 余志鹏 柳民意 张红强 崔燕[1,2] 余四九 樊江峰[1,2] QIAN Wen-Jie;DU Pei-Yan;LI Yi-Juan;ZHOU Ying-Cong;YAO Ying;LI Liu-Yang;YU Zhi-Peng;LIU Min-Yi;ZHANG Hong-Qiang;CUI Yan;YU Si-Jiu;FAN Jiang-Feng(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep&Goat,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]甘肃省牛羊胚胎工程技术研究中心,兰州730070

出　　处：《农业生物技术学报》2024年第6期1342-1352,共11页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(地区基金)(31660732);甘肃省科技重点研发计划(农业类)项目(20YF8NA131);陇原青年创新创业人才项目(2021LQGR47)。

摘　　要：Yes相关蛋白(Yes-associated protein,YAP)和TEA转录因子1(TEA domain transcription factor 1,TEAD-1)是Hippo信号通路的核心因子,在调控细胞增殖和器官生长方面发挥重要作用。为检测YAP和TEAD-1在牦牛(Bos grunniens)胎盘不同发育时期的表达,探究Hippo信号通路在调控胎盘生长发育方面的生理作用,本研究采集妊娠牦牛胎盘组织样品,以胎儿头臀距为依据,将其分为不同发育阶段(<2月龄,3月龄,4月龄,>5月龄),采用qRT-PCR、Western blot和免疫组织化学(immunohistochemistry,IHC)技术对YAP和TEAD-1的表达情况进行了检测分析。结果显示,在牦牛胎盘组织中,YAP和TEAD-1主要表达于胎儿绒毛膜滋养巨细胞、柱状滋养细胞和母体子宫肉阜隐窝上皮细胞。随着妊娠的进行,YAP和TEAD-1mRNA及其蛋白在牦牛胎盘组织中的表达量有明显的变化,且YAP和TEAD-1的变化趋势基本一致。这种协同表达特性及其表达量与胎盘发育阶段的相关性,提示YAP和TEAD-1可能通过Hippo信号通路参与了牦牛胎盘发育的调控,并在胎盘组织生理功能的发挥和妊娠维持方面发挥重要作用。研究结果为阐明牦牛胎盘发育及妊娠调控机制提供理论依据。Yes-associated protein(YAP)and TEA domain transcription factor 1(TEAD-1)are the core factors of the Hippo signaling pathway and play an important role in regulating cell proliferation and organ growth.In order to detect the expression of YAP and TEAD-1 in different developmental stages of yak(Bos grunniens)placenta,and to explore the physiological role of Hippo signaling pathway in regulating placental cell proliferation and organ growth regulation.In this study,placental tissue samples of pregnant yaks were collected and divided into different developmental stages(<2 months old,3 months old,4 months old,>5 months old)based on fetal head-to-hip distance.The expression of YAP and TEAD-1 was detected and analyzed by qRT-PCR,Western blot(WB)and Immunohistochemistry(IHC)technology were used to detect the expression of these genes.The results showed that YAP and TEAD-1 were mainly expressed in fetal chorionic trophoblast giant cells,columnar trophoblast cells and maternal placental crypt epithelial cells.With the progress of pregnancy,the expression of YAP and TEAD-1 mRNA and protein in yak placenta tissue change significantly,and the change trend of YAP and TEAD-1 was basically the same.The co-expression characteristics and the correlation between the expression level and the placental development stage suggested that YAP and TEAD-1 might be involved in the regulation of yak placental development through the Hippo signaling pathway,and play an important role in the physiological function of placental tissue and pregnancy maintenance.The results provides a theoretical basis for elucidating the mechanism of yak placental development and pregnancy regulation.

关 键 词：牦牛 胎盘 Yes相关蛋白(YAP) TEA转录因子1(TEAD-1) 

分 类 号：S857.21[农业科学—临床兽医学]