CRISPR/Cas9基因编辑技术在害虫基因功能及害虫防治研究中的应用进展  

Progress in the Application of CRISPR/Cas9 Gene Editing Technology in Pest Gene Function and Pest Control Research

作　　者：向云菊 张旺和 徐进 叶辉[3] XIANG Yun-Ju;ZHANG Wang-He;XU Jin;YE Hui(Yunnan Key Laboratory of Forest Disaster Warning and Control,Southwest Forestry University,Kunming 650224,China;Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Southwest Forestry University,Kunming 650224,China;School of Ecology and Environment,Yunnan University,Kunming 650091,China)

机构地区：[1]西南林业大学云南省森林灾害预警与控制重点实验室,昆明650224 [2]西南林业大学西南山地森林资源保育与利用教育部重点实验室,昆明650224 [3]云南大学生态与环境学院,昆明650091

出　　处：《农业生物技术学报》2024年第6期1415-1431,共17页Journal of Agricultural Biotechnology

基　　金：云南省基础研究重点项目(202201AS070025);云南省重点领域科技计划(202001BB050002);云南省森林灾害预警与控制重点实验室开放基金(ZKJS-S-202204)。

摘　　要：CRISPR/Cas9技术作为第3代基因编辑技术,凭借设计简单、操作方便快捷、使用成本低廉等特性,在生命科学研究领域掀起了一场技术革命。目前CRISPR/Cas9技术已成功应用于动物、植物及微生物等真核及原核生物的基因编辑;近年来,该技术也逐步被用于昆虫的研究中,并在害虫防治新技术探索方面显示了广阔的前景。本文主要就CRISPR/Cas9系统的工作原理进行了概述,并就该技术在害虫基因功能和防治方面的研究进展进行总结,旨在为利用该技术进行更为广阔的昆虫分子生物学及害虫防治策略研究提供参考。CRISPR/Cas9 technology,as the third generation of gene editing technology with simple design,convenient and quick operation,and low cost of use,has set off a technological revolution in the field of life sciences research.Currently,CRISPR/Cas9 has been successfully applied to gene editing in eukaryotic and prokaryotic organisms such as animals,plants and microorganisms.In recent years,CRISPR/Cas9 has also been used in the study of insects,and has shown great promise in the exploration of new technologies for pest control.This paper mainly provides an overview of the working principle of the CRISPR/Cas9 system and summarizes the progress of this technology in pest gene function and pest control.This review provides a reference for the use of CRISPR/Cas9 to carry out broader research in insect molecular biology and pest management strategies.

关 键 词：CRISPR/Cas9 基因编辑 脱靶效应 基因功能 害虫防治 基因驱动技术 

分 类 号：S433[农业科学—农业昆虫与害虫防治] Q78[农业科学—植物保护]