广西壮族自治区胸科医院不同年龄和性别肺泡灌洗液分枝杆菌菌种分布特征  

Distribution characteristics of Mycobacterium species in bronchoalveolar lavage fluid of different ages and genders in Guangxi Zhuang Automomous Region Chest Hospital

作　　者：蓝吉慧 唐柳生 雷振 董捷 覃善芳 黄丽玲 LAN Jihui;TANG Liusheng;LEI Zhen;DONG Jie;QIN Shanfang;HUANG Liling(Department of Clinical Laboratory,Guangxi Zhuang Autonomous Region Chest Hospital,Guangxi Zhuang Autonomous Region,Liuzhou545005,China;Department of Science and Education Division,Guangxi Zhuang Autonomous Region Chest Hospital,Guangxi Zhuang Autonomous Region,Liuzhou545005,China)

机构地区：[1]广西壮族自治区胸科医院检验科,广西柳州545005 [2]广西壮族自治区胸科医院科教科,广西柳州545005

出　　处：《中国当代医药》2024年第16期122-125,共4页China Modern Medicine

基　　金：“十三五”国家科技重大专项项目(2018ZX10715008-002);广西医疗卫生适宜技术开发与推广应用项目(S2022042)。

摘　　要：目的通过微阵列基因芯片法检测肺泡灌洗液分枝杆菌,了解广西壮族自治区胸科医院肺部感染分枝杆菌患者不同年龄和性别的菌种分布特征。方法收集2018年1月至2022年12月广西壮族自治区胸科医院收治的肺部感染患者的肺泡灌洗液通过微阵列基因芯片法检测的结果,统计就诊患者的资料,分析其分枝杆菌的分布特征。结果949份灌洗液检测出分枝杆菌348例,检测率为36.7%(348/949),其中结核分枝杆菌复合群占83.6%(291/348),男性检出率为33.2%(203/611),女性检出率为26.0%(88/338),男女间检出率比较,差异有统计学意义(χ^(2)=5.29,P<0.05),非结核分枝杆菌(NTM)占16.4%(57/348),男性检出率为5.1%(31/611),女性检出率为7.7%(26/338),男女间检出率比较,差异无统计学意义(χ^(2)=2.64,P>0.05)。检测出的NTM中,胞内分枝杆菌占35.1%(20/57),龟/脓肿分枝杆菌占33.3%(19/57),鸟分枝杆菌占19.3%(11/57),戈登分枝杆菌占5.3%(3/57),蟾蜍分枝杆菌占5.3%(3/57),浅黄分枝杆菌占1.7%(1/57)。各年龄段的肺部感染患者中,结核分枝杆菌复合群的检出率比较,差异无统计学意义(χ^(2)=10.8,P>0.05),各年龄段的肺部感染患者中,NTM的检出率比较,差异有统计学意义(χ^(2)=18.7,P<0.01)。结论广西壮族自治区胸科院肺部感染分枝杆菌以结核分枝杆菌复合群为主,NTM主要的流行菌株为胞内分枝杆菌、龟/脓肿分枝杆菌和鸟分枝杆菌,结核分枝杆菌复合群的肺部感染患者不存在年龄差异,但存在性别差异,NTM的肺部感染患者不存在性别差异,但存在年龄差异,有老龄化趋势,NTM菌种具有多样化。Objective Detection of Mycobacterium species in alveolar lavage fluid using microarray gene chip method to understand the distribution characteristics of Mycobacterium species in patients with pulmonary infection in Guangxi Zhuang Autonomous Region Chest Hospital at different ages and genders.Methods The results of alveolar lavage fluid detection using microarray gene chip method from patients with pulmonary infections admitted to Guangxi Zhuang Automomous Region Chest Hospital from January 2018 to December 2022 were colletced,and the distribution characteristics of Mycobacteria in the patients was analyzed.Results A total of 348 cases of Mycobacterium tuberculosis were detected from 949 lavage fluid samples,with a detection rate of 36.7%(348/949).Among them,the Mycobacterium tuberculosis complex group accounted for 83.6%(291/348),with a detection rate of 33.2%(203/611)in males and 26.0%(88/338)in females.The difference in detection rates between males and females was statistically significant(χ^(2)=5.29,P<0.05),non tuberculosis Mycobacteria(NTM)accounted for 16.4%(57/348),with the male detection rate was 5.1%(31/611),and the female detection rate was 7.7%(26/338),and there was no statistically significant difference in the detection rate between males and females(χ^(2)=2.64,P>0.05).In the detected NTM,intracellular Mycobacterium accounted for 35.1%(20/57),Mycobacterium chelonae/abscess accounted for 33.3%(19/57),My cobacterium avium accounted for 19.3%(11/57),Gordon Mycobacterium accounted for 5.3%(3/57),toad Mycobacterium accounted for 5.3%(3/57),and light yellow Mycobacterium accounted for 1.7%(1/57).There was no statistically significant difference in age groups among lung infection patients who have detected a complex of Mycobacterium tuberculosis(χ^(2)=10.8,P>0.05),there was a statistically significant difference in the age groups of lung infection patients who detected NTM(χ^(2)=18.7,P<0.01).Conclusion The pulmonary infection of Mycobacterium tuberculosis in Guangxi Zhuang Automomous Region Chest Hospi

关 键 词：微阵列基因芯片法 分枝杆菌 肺泡灌洗液 检出率 

分 类 号：R563[医药卫生—呼吸系统]