EZH2参与TLR介导的B细胞增殖和抗体分泌  

EZH2 is involved in TLR-mediated B cell proliferation and antibody secretion

作　　者：苏晓亚 吴静[2] 陈同辛[1,2] 洪莉[2,3] SU Xiao-ya;WU Jing;CHEN Tong-xin;HONG Li(Department of Allergy and Immunology,Shanghai Children's Medical Center,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China;Pediatric Translational Medicine Institute,Shanghai Children's Medical Center,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China;Department of Clinical Nutrition,Shanghai Children's Medical Center,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China)

机构地区：[1]上海交通大学医学院附属上海儿童医学中心过敏免疫科,上海200127 [2]上海交通大学医学院附属上海儿童医学中心儿科转化医学研究所,上海200127 [3]上海交通大学医学院附属上海儿童医学中心临床营养科,上海200127

出　　处：《现代免疫学》2024年第3期214-220,共7页Current Immunology

基　　金：国家自然科学基金(81871303)。

摘　　要：TLR属于模式识别受体家族中的一员,能够识别多种类型的PAMP。TLR信号通路在B细胞分化和激活过程中起着多种作用。为研究zeste基因的增强子同源物2(enhancer of zeste homolog 2,EZH2)在B细胞TLR介导的免疫反应中的作用,调取国家基因组科学数据中心与人类B细胞TLR信号通路有关的转录组数据,初步分析多梳抑制复合体2(polycomb repressive complex 2,PRC2)亚基的表达改变。随后分离人PBMC中的B细胞,用qRT-PCR和Western blotting的方法从转录水平和蛋白水平检测B细胞TLR通路激活后EZH2的表达水平。结果显示B细胞TLR激活后EZH2的表达增加。接着用EZH2抑制剂3-去氮腺嘌呤(3-deazaneplanocin A,DZNep)预处理人PBMC细胞,用流式细胞术和ELISA检测TLR介导的B细胞增殖和抗体分泌的改变。结果显示抑制B细胞中EZH2的功能后,TLR9介导的B细胞增殖和抗体分泌减少。综上,EZH2能够参与B细胞TLR介导的免疫反应,抑制B细胞中EZH2的表达后,TLR介导的B细胞增殖和抗体分泌减少。TLR is a member of the pattern recognition receptor family,which recognizes many types of PAMP.TLR signaling plays multiple roles in B-cell differentiation and activation.To investigate the role of enhancer of zeste homolog 2(EZH2)in TLR-mediated immune response of B cells,transcriptomic data related to TLR signaling pathway of human B cells were collected from National Genome Science Data Center and expression changes of polycomb repressive complex 2(PRC2)subunits were analyzed.EZH2 expression in PBMC after activation via TLR pathway was detected by qRT-PCR and Western blotting.The results showed that EZH2 expression increased after TLR activation in B cells.Next,human PBMC were pretreated with EZH2 inhibitor 3-deazaneplanocin A(DZNep),and TLR-mediated changes in B cell proliferation and antibody secretion were detected by flow cytometry and ELISA.The results showed that inhibition of EZH2 in B cells resulted in a decrease in TLR9-mediated B cell proliferation and antibody secretion.In conclusion,EZH2 participates in the TLR-mediated immune response of B cells.Inhibition of EZH2 expression in B cells leads to a decrease in TLR-mediated B cell proliferation and antibody secretion.

关 键 词：TOLL样受体 Zeste基因增强子同源物2 B淋巴细胞 增殖 抗体分泌 

分 类 号：R392.11[医药卫生—免疫学]