大豆GmbZIP27基因的克隆及表达分析  

Cloning and Expression Analysis of Soybean GmbZIP27 Gene

作　　者：胡浩 鹿瑞昭 王怡 倪志勇[2] 于月华[1] Hu Hao;Lu Ruizhao;Wang Yi;Ni Zhiyong;Yu Yuehua(College of Agronomy,Xinjiang Agricultural University,Urumqi,830052;College of Life Sciences,Xinjiang Agricultural University,Urumqi,830052)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业大学生命科学学院,乌鲁木齐830052

出　　处：《分子植物育种》2024年第11期3481-3486,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31860295,32160446);新疆维吾尔自治区自然科学基金项目(2021D01A93);新疆维吾尔自治区天山创新团队计划项目(2020D14002)共同资助。

摘　　要：bZIP蛋白是一类调控植物生长发育和抗性的转录因子。为探究大豆bZIP的生物学功能,利用PCR方法从大豆中克隆到1个bZIP基因GmbZIP27,对该基因进行生物信息学、表达模式和结合特异性分析。结果表明,GmbZIP27基因的开放阅读框长为825 bp,编码274个氨基酸,蛋白质分子质量为30.426 kD,等电点为8.54,含组氨酸和丝氨酸活性位点,为不稳定疏水蛋白,二级结构以α-螺旋和β-折叠为主。亚细胞定位分析发现GmbZIP27定位于细胞核。酵母单杂交实验表明GmbZIP27不能结合GmFBX176启动子中的ABRE元件。组织特异性表达模式表明,GmbZIP27在大豆组织中的相对表达量由高到低依次为子叶、根、叶和茎,推测GmbZIP27基因参与调控大豆的生长发育过程。本研究为深入解析bZIP转录因子在大豆发育及非生物胁迫应答中的功能提供一定理论依据。The bZIP proteins are a class of transcription factors that regulate plant growth and resistance.In order to explore the biological function of bZIP in soybean,a bZIP gene GmbZIP27 was cloned from soybean by PCR,and the bioinformatics,expression pattern and binding specificity of the gene were analyzed.The results showed that GmbZIP27 was an unstable hydrophobic protein with an open reading frame length of 825 bp,encoding 274amino acids,a molecular weight of 30.426 kD,and an isoelectric point of 8.54.It contained active sites of histidine and serine,and its secondary structure was dominated byα-helix andβ-fold.Subcellular localization analysis revealed that GmbZIP27 was located in the nucleus.Yeast one-hybrid showed that GmbZIP27 could not bind to the ABRE element in the promoter GmFBX176.Expression pattern analysis showed that GmbZIP27 could be expressed in all the detected tissues,and the expression level was successively from high to low in cotyledon,root,leaf and stem,indicating that GmbZIP27 might be involved in the regulation of soybean growth and development.These study provide a theoretical basis for further analysis of the functions of bZIP transcription factors in soybean development and abiotic stress response.

关 键 词：大豆 GmbZIP27 酵母单杂交 表达模式分析 

分 类 号：Q94[生物学—植物学]