伞形科四种药用植物鲨烯合酶的生物信息学分析  

Bioinformatics Analysis of Squalene Synthase from Four Medicinal Plants of Apiaceae

作　　者：张丽 杨娜 关鑫 张武[1] 王长宝 Zhang Li;Yang Na;Guan Xin;Zhang Wu;Wang Changbao(College of Science,Jiamusi University,Jiamusi,154007;College of Biology and Pharmacy,Yulin Normal University,Yulin,537000)

机构地区：[1]佳木斯大学理学院,佳木斯154007 [2]玉林师范学院生物与制药学院,玉林537000

出　　处：《分子植物育种》2024年第11期3554-3562,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(81441132);国家标本平台教学标本子平台运行服务项目子课题(2005DKA21403-JK);玉林师范学院高层次人才引进项目(G2019ZK38)共同资助。

摘　　要：鲨烯合酶(squalene synthase,SQS)是三萜类化合物合成的关键调控酶。本研究利用生物信息学软件,从蛋白质理化性质、亚细胞定位,蛋白质结构和系统发育关系等方面分析了伞形科四种药用植物的SQS序列。结果表明:除积雪草外,其余植物的SQS均为稳定蛋白;所有SQS序列都存在明显的疏水区/亲水区,属亲水蛋白;全部SQS序列主要定位于细胞质和质膜,均未发现信号肽,但C端均存在2处跨膜区域;二级结构和功能域均以无规则卷曲和α-螺旋为主要结构元件;系统发育分析与物种的分类关系是一致的。这些结果为深入认识鲨烯合酶结构与功能的关系及其在伞形科植物中的分子进化提供了科学依据。Squalene synthase(SQS)is a key rate limiting enzyme in triterpenoid biosynthesis pathway.In this study,bioinformatics software tools were used to predict and analyze physicchemical properties,subcellular localization,protein structure and phylogenetic relationship of seven SQS sequences from four Apiaceae medicinal plants.The results showed that SQS proteins were stable except that of Centella asiatica L..All SQS have obvious hydrophobic/hydrophobic regions,belonging to hydrophilic protein.SQS were mainly localized in the cytoskeleton and cytoplasm,and signal peptide were not detected.However,there were two transmembrane regions in the C-terminal.The secondary structure and functional domains were dominated by random coil andα-helix.The result of phylogenetic analysis was also consistent with that of plant taxonomic relationship.These results provide a scientific basis for deep understanding of the relationship between structure and function of SQS,and its molecular evolution in Apiaceaae.

关 键 词：伞形科 鲨烯合酶 生物信息学分析 

分 类 号：R28[医药卫生—中药学]