黑骨藤主要化学成分在正常和类风湿性关节炎大鼠血浆、尿液、粪便中的代谢产物分析  

Analysis of metabolites of the main chemical components of Periploca forrestii in normal and rheumatoid arthritis rats’plasma,urine and feces

作　　者：张熊莉 夏涛 郑林[1] 迟明艳 李月婷[1] 巩仔鹏[1] 金阳 刘亭[1] 黄勇[1] ZHANG Xiong-li;XIA Tao;ZHENG Lin;CHI Ming-yan;LI Yue-ting;GONG Zi-peng;JIN Yang;LIU Ting;HUANG Yong(Guizhou Provincial Key Laboratory of Pharmaceutics,State Key Laboratory of Functions and Applications of Medicinal Plants,Guiyang 550004,China;Guizhou Medical University of Pharmacy,Guiyang 550004,China;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM(Ministry of Education),Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室/药用植物功效与利用国家重点实验室,贵阳550004 [2]贵州医科大学药学院,贵阳550004 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵阳550004

出　　处：《药物分析杂志》2024年第4期594-602,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金项目(U1812403、81660691);贵州省科技计划项目(黔科合平台人才-GCC[2022]031-1、CXTD[2023]019);贵州省教育厅自然科学研究项目(黔教合KY字[2021]033)。

摘　　要：目的:研究黑骨藤在正常及佐剂性关节炎(AA)大鼠体内的代谢产物,探究类风湿性关节炎(RA)对黑骨藤有效成分在大鼠体内代谢的影响。方法:采用弗氏完全佐剂制备AA大鼠模型;运用UPLC-Q TOF MSE方法,采用ACQUITY UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)色谱柱,以0.01%甲酸水-0.01%甲酸乙腈为流动相梯度洗脱,流速0.25 mL·min^(-1),采用电喷雾离子源,在负离子模式下,对正常和AA模型大鼠灌服黑骨藤提取物后的血浆、尿液和粪便进行分析。结果:在正常大鼠体内和AA模型大鼠体内分别检测到2、3个原型成分,32、35个代谢产物,代谢途径主要包括单咖啡酰基奎宁酸还原、甲基化、开环裂解、葡萄糖醛酸化,二咖啡酰基奎宁酸还原、甲基化、乙酰化、异构化、硫酸酯化、葡萄糖醛酸化等。结论:AA模型大鼠血浆和尿液中的代谢产物比正常大鼠更具多样性,RA疾病状态可能会影响黑骨藤有效成分在体内的代谢途径。Objective:To investigate the metabolites of Periploca forrestii in plasma,urine,and feces of normal and adjuvant arthritis(AA)rats,and explore the effect of rheumatoid arthritis(RA)on the metabolism of active components of P.forrestii.Methods:AA rat model was made by means of Freund’s complete adjuvant.Plasma,urine and feces of normal and AA rats were analyzed by UPLC-Q-TOF-MS E method using ACQUITY UPLC BEH C_(18)(100 mm×2.1 mm,1.7μm)column with 0.01%formic acid water-0.01%formic acid acetonitrile as mobile phase gradient elution at a flow rate of 0.25 mL·min^(-1),and electrospray ion source under negative ion mode.Results:Two and three prototype components,32 and 35 metabolites were detected in normal rats and AA model rats.The metabolic pathways mainly include monocaffeoylquinic acid reduction,methylation,ring-open⁃ing cleavage,glucuronidation,dicaffeoylquinic acid reduction,methylation,acetylation,isomerization,sulfation glucuronidation,etc.Conclusion:The metabolites in plasma and urine of AA model rats are more diverse than those in normal rats,and the disease state of RA may affect the metabolic pathway of effective components of P.forrestii in the body.

关 键 词：类风湿性关节炎 黑骨藤提取物 单咖啡酰基奎宁酸 二咖啡酰基奎宁酸 代谢产物 超高效液相色谱-串联电喷雾飞行时间质谱 代谢途径 

分 类 号：R917[医药卫生—药物分析学]