基于指纹图谱和含量测定评价地黄不同入药形式制千金黄连丸质量  

Quality evaluation of Qianjin Huanglian Pills with different medicinal forms of Rehmanniae Radix based on fingerprints and components determination

作　　者：张春亚 张威 李莹莹[1] 雷敬卫[1] 杨颜溶 田瀚举 张娟[1] 赵新梅 段浩瀚 龚海燕[1] 杨春静[3] 王笑笑 ZHANG Chunya;ZHANG wei;LI Yingying;LEI Jingwei;YANG Yanrong;TIAN Hanju;ZHANG Juan;ZHAO Xinmei;DUAN Haohan;GONG Haiyan;YANG Chunjing;WANG Xiaoxiao(Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Zhengzhou Health Vocational College,Zhengzhou 450122,China;The Third Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]郑州卫生健康职业学院,河南郑州450122 [3]河南中医药大学第三附属医院,河南郑州450046

出　　处：《中草药》2024年第7期2227-2236,共10页Chinese Traditional and Herbal Drugs

基　　金：河南省中医药科学研究专项(2023ZY3037)。

摘　　要：目的综合评价地黄不同入药形式制千金黄连丸(Qianjin Huanglian Pills,QHP)的质量。方法建立地黄不同入药形式制QHP的HPLC指纹图谱,通过多元统计分析及2种环烯醚萜苷类成分(梓醇、益母草苷)、5种生物碱类成分(黄连碱、表小檗碱、药根碱、小檗碱、巴马丁)、5种糖类成分(果糖、葡萄糖、蔗糖、棉子糖、水苏糖)定量分析对30批地黄不同入药形式制QHP的质量进行评价。结果30批QHP样品指纹图谱相似度较高,采用SPSS19.0和SIMCA-P14.1软件对黄连丸19个共有峰峰面积进行系统聚类分析和主成分分析,30批样品可按鲜地黄连丸、鲜干黄连丸、生地黄连丸明显分为3类;采用正交偏最小二乘法-判别分析标记了黄连丸中8个差异性成分,分别为峰1、6、15(表小檗碱)、3、4、2(梓醇)、16、9。含量测定结果表明,2种环烯醚萜苷类成分梓醇、益母草苷总含量顺序为鲜干黄连丸>鲜地黄连丸>生地黄连丸,5种生物碱类成分总含量顺序为生地黄连丸>鲜地黄连丸>鲜干黄连丸,5种糖类成分总含量顺序为鲜地黄连丸>鲜干黄连丸>生地黄连丸,棉子糖和水苏糖的含量均值顺序为鲜地黄连丸>鲜干黄连丸>生地黄连丸,提示鲜地黄连丸、鲜干黄连丸中梓醇、益母草苷、棉子糖、水苏糖含量较高,鲜地黄连丸、鲜干黄连丸中生物碱类成分含量略低于生地黄连丸。结论建立的方法操作简便、重复性好,可用于QHP的定性定量分析;研究结果可为完善QHP质量标准提供依据。Objective To comprehensively evaluate the quality of Qianjin Huanglian Pills(千金黄连丸,QHP)with different medicinal forms of Dihuang(Rehmanniae Radix,RR).Methods The HPLC fingerprints of QHW with different medicinal forms of RR were established.The quality of 30 batches of QHP was evaluated by multivariate statistical analysis and the quantitative analysis of two iridoid glycosides(catalpol,leonuride),five alkaloids(coptisine,epiberberine,jatrorrhizine,berberine,palmatine),and five carbohydrate components(fructose,glucose,sucrose,raffinose,stachyose).Results The fingerprints of 30 batches of QHP showed a high similarity.In addition,SPSS 19.0 and SIMAC-P14.1 were used for hierarchical cluster analysis(HCA)and principal component analysis(PCA)of the 19 common peak areas of QHP HPLC fingerprints,and the 30 batches of samples were divided into there categories:Xiandi Huanglian Pills(鲜地黄连丸,XD),Xiangan Huanglian Pills(鲜干黄连丸,XG)and Shengdi Huanglian Pills(生地黄连丸,SD).Eight differential components in QHP were marked by orthogonal partial least squares discrimination analysis(OPLS-DA),which were chromatographic peaks 1,6,15(epiberberine),3,4,2(catalpol),16 and 9.The results of content determination showed that the total content of the two iridoid glycosides were in the order of XG>XD>SD,the total content of the five alkaloids were in the order of SD>XD>XG,the total content of the five carbohydrate components were in the order of XD>XG>SD.The average content of raffinose and stachyose were in the order of XD>XG>SD,suggesting that XD and XG contained higher contents of catalpol,leonuride,raffinose and stachyose.XD and XG contained slightly lower contents of alkaloids than SD.Conclusion The method established in this study is simple and reproducible,and can be used for the qualitative and quantitative analysis of QHP,which can provide a basis for improving the quality standard of QHP.

关 键 词：地黄 千金黄连丸 指纹图谱 梓醇 益母草苷 黄连碱 表小檗碱 药根碱 小檗碱 巴马丁 果糖 葡萄糖 蔗糖 棉子糖 水苏糖 

分 类 号：R283.6[医药卫生—中药学]