Spontaneous mutations and mutational responses to penicillin treatment in the bacterial pathogen Streptococcus pneumoniae D39  

作　　者：Wanyue Jiang Tongtong Lin Jiao Pan Caitlyn ERivera Clayton Tincher Yaohai Wang Yu Zhang Xiang Gao Yan Wang Ho‑Ching T.Tsui Malcolm E.Winkler Michael Lynch Hongan Long 

机构地区：[1]Institute of Evolution and Marine Biodiversity,KLMME,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Biology and Biotechnology,Laoshan Laboratory,Qingdao 266237,China [3]Department of Biology,Indiana University,Bloomington,IN 47405,USA [4]School of Mathematics Science,Ocean University of China,Qingdao 266000,China [5]State Key Laboratory of Microbial Technology,Microbial Technology Institute,School of Life Science,Shandong University,Qingdao 266237,China [6]Biodesign Center for Mechanisms of Evolution,Arizona State University,Tempe,AZ 85281,USA

出　　处：《Marine Life Science & Technology》2024年第2期198-211,共14页海洋生命科学与技术（英文）

基　　金：financially supported by Laoshan Laboratory(LSKJ202203203);the National Natural Science Founda-tion of China(31961123002,32270435);the Fundamental Research Funds for the Central Universities of China(202041001);the Shan-dong Provincial Natural Science Foundation(ZR2023QC191);the Postdoctoral Fellowship Program of CPSF under Grant Number GZC20232504;the Multidisciplinary University Research Initiative Award from the US Army Research Office(W911NF-09-1-0444);National Institutes of Health award(R35-GM122566 to ML)and(R35-GM131767 to MEW).

摘　　要：Bacteria with functional DNA repair systems are expected to have low mutation rates due to strong natural selection for genomic stability.However,our study of the wild-type Streptococcus pneumoniae D39,a pathogen responsible for many common diseases,revealed a high spontaneous mutation rate of 0.02 per genome per cell division in mutation-accumulation(MA)lines.This rate is orders of magnitude higher than that of other non-mutator bacteria and is characterized by a high mutation bias in the A/T direction.The high mutation rate may have resulted from a reduction in the overall efficiency of selection,conferred by the tiny effective population size in nature.In line with this,S.pneumoniae D39 also exhibited the lowest DNA mismatch-repair(MMR)efficiency among bacteria.Treatment with the antibiotic penicillin did not elevate the mutation rate,as penicillin did not induce DNA damage and S.pneumoniae lacks a stress response pathway.Our findings suggested that the MA results are applicable to within-host scenarios and provide insights into pathogen evolution.

关 键 词：Neutral evolution Mutation spectrum ANTIBIOTICS DNA mismatch repair Oxidative damage repair 

分 类 号：Q178.53[生物学—水生生物学]