Dominance of transposable element‑related satDNAs results in great complexity of“satDNA library”and invokes the extension towards“repetitive DNA library”  

作　　者：Monika Tunjić‑Cvitanić Daniel García‑Souto Juan J.Pasantes EvaŠatović‑Vukšić 

机构地区：[1]Division of Molecular Biology,Ruđer BoškovićInstitute,10000 Zagreb,Croatia [2]Genomes and Disease,Centre for Research in Molecular Medicine and Chronic Diseases(CIMUS),Universidade de Santiago de Compostela,15706 Santiago de Compostela,Spain [3]Department of Zoology,Genetics and Physical Anthropology,Universidade de Santiago de Compostela,15706 Santiago de Compostela,Spain [4]Centro de Investigación Mariña,Dpto de Bioquímica,Xenética e Inmunoloxía,Universidade de Vigo,36310 Vigo,Spain

出　　处：《Marine Life Science & Technology》2024年第2期236-251,共16页海洋生命科学与技术（英文）

基　　金：supported by:the Croatian Science Foundation,grant number IP-2019-045522;Xunta de Galicia(ED431C 2020/05)and the European Union(Euro-pean Regional Development Fund-ERDF);Xunta de Galicia(Centro singular de investigación de Galicia,accreditation 2019-2022)and the European Union(European Regional Development Fund-ERDF);financially supported by Xunta de Galicia,a postdoctoral contract ED481B/2018/091.

摘　　要：Research on bivalves is fast-growing,including genome-wide analyses and genome sequencing.Several characteristics qualify oysters as a valuable model to explore repetitive DNA sequences and their genome organization.Here we char-acterize the satellitomes of five species in the family Ostreidae(Crassostrea angulata,C.virginica,C.hongkongensis,C.ariakensis,Ostrea edulis),revealing a substantial number of satellite DNAs(satDNAs)per genome(ranging between 33 and 61)and peculiarities in the composition of their satellitomes.Numerous satDNAs were either associated to or derived from transposable elements,displaying a scarcity of transposable element-unrelated satDNAs in these genomes.Due to the non-conventional satellitome constitution and dominance of Helitron-associated satDNAs,comparative satellitomics demanded more in-depth analyses than standardly employed.Comparative analyses(including C.gigas,the first bivalve species with a defined satellitome)revealed that 13 satDNAs occur in all six oyster genomes,with Cg170/HindIII satDNA being the most abundant in all of them.Evaluating the“satDNA library model”highlighted the necessity to adjust this term when studying tandem repeat evolution in organisms with such satellitomes.When repetitive sequences with potential variation in the organizational form and repeat-type affiliation are examined across related species,the introduction of the terms“TE library”and“repetitive DNA library”becomes essential.

关 键 词：Satellitome Comparative satellitomics Helitron Bivalves “Dark matter of the genome” 

分 类 号：Q78[生物学—分子生物学]