Mucosal immune responses to Ichthyophthirius multifiliis in the ocular mucosa of rainbow trout(Oncorhynchus mykiss,Walbaum),an ancient teleost fish  

作　　者：Weiguang Kong Guangyi Ding Gaofeng Cheng Peng Yang Zhen Xu 

机构地区：[1]Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China [2]Department of Aquatic Animal Medicine,College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Marine Life Science & Technology》2024年第2期266-279,共14页海洋生命科学与技术（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(32225050,32073001,32303053);the National Key R&D Program of China(2022YFF1000302);the China Postdoctoral Science Foundation(2022M713325,2023T160671).

摘　　要：The eye,as a specialized visual organ,is directly exposed to the external environment,and,therefore,it faces constant chal-lenges from external pathogenic organisms and toxins.In the ocular mucosa(OM)of mammals,mucosal-associated lymphoid tissues(MALTs)constitute the primary line of defense.However,the immune defense role of the OM remains unknown in aquatic vertebrates.To gain insights into the immune processes within the OM of teleost fish,we developed an infection model of rainbow trout(Oncorhynchus mykiss)OM using a parasite,Ichthyophthirius multifiliis(Ich).Immunofluorescence,qPCR,and H&E staining revealed that Ich successfully infiltrates the OM of rainbow trout,leading to pathological struc-tural changes,as evidenced by A&B staining.Importantly,the qPCR results indicate an up-regulation of immune-related genes following Ich infection in the OM.Moreover,transcriptome analyses were conducted to detect immune responses and impairments in eye function within the OM of rainbow trout with Ich infection.The results of the transcriptome analysis that Ich infection can cause an extensive immune response in the OM,ultimately affecting ocular function.To the best of our knowledge,our findings represent for the first time that the teleost OM could act as an invasion site for parasites and trigger a strong mucosal immune response to parasitic infection.

关 键 词：Ocular mucosa Mucosal immunity Transcriptome analysis Oncorhynchus mykiss Ichthyophthirius multifiliis 

分 类 号：S917.4[农业科学—水产科学]