Morphological and molecular analyses of a Philine kinglipini outbreak in summer of 2022 in Jiaozhou Bay,China  

作　　者：Zelin DUAN Yanan YU Jing WANG Junlong ZHANG Nansheng CHEN 

机构地区：[1]CAS Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [2]Laboratory of Marine Ecology and Environmental Science,Laoshan Laboratory,Qingdao 266237,China [3]Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China [4]Laboratory of Marine Organism Taxonomy and Phylogeny,Qingdao Key Laboratory of Marine Biodiversity and Conservation,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [5]Marine Biological Museum,Chinese Academy of Sciences,Qingdao 266071,China [6]Department of Molecular Biology and Biochemistry,Simon Fraser University,Burnaby,British Columbia,V5A 1S6,Canada

出　　处：《Journal of Oceanology and Limnology》2024年第3期831-848,共18页海洋湖沼学报（英文）

基　　金：Supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Nos.XDA22050302,XDB42000000);the Taishan Scholar Project Special Fund(to Nansheng CHEN);the Post-doctoral Program of Qingdao 2021(No.E2KY14101N)。

摘　　要：A sudden and unprecedented outbreak of molluscan Philine kinglipini occurred in summer 2022 in Jiaozhou Bay,Shandong,China,causing substantial damage to local mariculture industry of the Manila clam Ruditapes philippinarum.Although P.kinglipini has been found in many coastal regions of China,the molecular research of P.kinglipini has not been extensively studied,making it difficult to accurately identify and track P.kinglipini samples in field using molecular methods.Samples were collected during the outbreak and their morphological features and molecular sequences were analyzed.Results show that the causative species of the outbreak was P.kinglipini.The mitochondrial genome(mt DNA)of P.kinglipini was constructed for the first time,based on which phylogenetic analysis of the mt DNAs of P.kinglipini and related species in the order Cephalaspidea was carried out.As revealed by metabarcoding analysis of 18S rDNA V4,the seasonal change of P.kinglipini and closely related Philine species was striking with peaks between April and August.Therefore,metabarcoding analysis is applicable tool for monitoring the bloom development of P.kinglipini and related species.This study generated for the first time essential molecular marker sequences and mtDNA of P.kinglipini,which provided a reference for future characterization and monitoring of its outbreaks and for phylogenetic analysis of Philine species.

关 键 词：marine species outbreaks metabarcoding analysis mitochondrial genome Philine kinglipini PHYLOGENETICS spatial-temporal dynamics 

分 类 号：S968.31[农业科学—水产养殖]