Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle  

作　　者：Ye ZHAO Han WANG Haona WANG Lijie ZHANG Hao SUN Yanying ZHANG 

机构地区：[1]Ocean School,Yantai University,Yantai 264005,China

出　　处：《Journal of Oceanology and Limnology》2024年第3期849-864,共16页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(No.41906098);the NSFC-Shandong Joint Fund(No.U2106208);the Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province(No.2022KJ269)。

摘　　要：Sea cucumber Apostichopus japonicus is an ideal model organism for marine invertebrate aestivation;it annually enters a“sleeping state”for nearly 3 months when ambient water temperature range is 25–30℃.The natural fasting is accompanied by remodeling the intestinal biota and function,which is a part of host biology and could affect the gut microbiota.We investigatesd the impact of annual aestivation on gut microbiota using high-throughput sequencing of 16S rRNA amplicons.Results reveal a notable alteration in the composition of gut bacteria during aestivation during which various indigenous families and genera that exhibit a preference for dietary glycans(e.g.,family Rhodobacteraceae and Flavobacteriaceae)decreased,while the taxa capable of utilizing substrates derived from the host(e.g.,genus Akkermansia and Prevotella)increased,and so did certain opportunistic pathogenic bacteria.Moreover,the investigation delved into the gut morphology and immunity response of A.japonicus and reveal that the intestine of A.japonicus undergoes substantial atrophy and degeneration during aestivation.However,there was an observed augmentation in the levels of acid and neutral mucin within mucous cells,as well as an enhanced immune defense response(as evidenced by increased gene expression of AjTLR3,LITAF,Ajp105,and LYZ).These results imply that the composition of sea cucumber gut microbiota differed between aestivation and active periods,which potentially affects the intestinal functions of the host and the symbiotic relationship between host and its microbiota over the activeaestivation cycle.

关 键 词：Apostichopus japonicus AESTIVATION gut microbiota DIET Illumina sequencing 

分 类 号：S917.4[农业科学—水产科学]