Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber  

作　　者：Yanan LI Zongfu CHEN Haibin ZHANG Ruoyu LIU Shuichun CHEN Li LIN 

机构地区：[1]Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding,College of Animal Sciences and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510222,China [2]Institute of Deep-sea Science and Engineering,Chinese Academy of Sciences,Sanya 572000,China [3]College of Marine Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China [4]Zhanjiang Tianli Sea Cucumber New Technology Development Co.,Ltd.,Zhanjiang 524000,China

出　　处：《Journal of Oceanology and Limnology》2024年第3期893-904,共12页海洋湖沼学报（英文）

基　　金：Supported by the Guangdong Province Basic and Applied Basic Research Fund Project(No.2020A1515110826);the National Natural Science Foundation of China(No.42006115);the Major Scientific and Technological Projects of Hainan Province(No.ZDKJ2021036)。

摘　　要：Manganese superoxide dismutase(MnSOD)is an antioxidant that exists in mitochondria and can effectively remove superoxide anions in mitochondria.In a dark,high-pressure,and low-temperature deep-sea environment,MnSOD is essential for the survival of sea cucumbers.Six MnSODs were identified from the transcriptomes of deep and shallow-sea sea cucumbers.To explore their environmental adaptation mechanism,we conducted environmental selection pressure analysis through the branching site model of PAML software.We obtained night positive selection sites,and two of them were significant(97F→H,134K→V):97F→H located in a highly conservative characteristic sequence,and its polarity c hange might have a great impact on the function of MnSOD;134K→V had a change in piezophilic a bility,which might help MnSOD adapt to the environment of high hydrostatic pressure in the deepsea.To further study the effect of these two positive selection sites on MnSOD,we predicted the point mutations of F97H and K134V on shallow-sea sea cucumber by using MAESTROweb and PyMOL.Results show that 97F→H,134K→V might improve MnSOD’s efficiency of scavenging superoxide a nion and its ability to resist high hydrostatic pressure by moderately reducing its stability.The above results indicated that MnSODs of deep-sea sea cucumber adapted to deep-sea environments through their amino acid changes in polarity,piezophilic behavior,and local stability.This study revealed the correlation between MnSOD and extreme environment,and will help improve our understanding of the organism’s adaptation mechanisms in deep sea.

关 键 词：HOLOTHUROIDEA environmental adaptation positive selection point mutation 

分 类 号：S917.4[农业科学—水产科学]