不同持水量和氮肥形态对丛枝菌根真菌调节柑橘幼苗生长和氮积累的影响  

Effects of different water holding capacity and nitrogen fertilizer forms on the regulation of arbuscular mycorrhizal fungi on the growth and nitrogen accumulation of citrus seedlings

作　　者：李京晨 王曙光[1] 郭佩佩 张丽[1] Li Jingchen;Wang Shuguang;Guo Peipei;Zhang Li(College of Tropical Crops,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学热带作物学院,海南海口570228

出　　处：《海南大学学报（自然科学版）》2024年第2期130-137,共8页Natural Science Journal of Hainan University

基　　金：海南省自然科学基金(320RC497);海南大学科研启动基金(KYQD(ZR)1978)。

摘　　要：以柑橘幼苗为研究对象,采用分室(分生长室与菌丝室)根箱装置,比较了不同水分处理[60%WFPS(Water-filled pore space);80%WFPS]与两种氮肥形态(NH_(4)^(+);NO_(3)^(-))下,丛枝菌根真菌(CK:无菌丝无根系;AMF:有菌丝无根系;RAMF:有菌丝有根系)对土壤氮含量、植株生物量和氮积累的影响.结果表明:水分水平、氮肥形态和丛枝菌根真菌均显著影响土壤氮含量、柑橘幼苗生物量及植株氮积累量;与60%田间持水量相比,80%田间持水量的土壤硝态氮和总有效氮含量分别降低了26.42%和8.28%,柑橘地上部和总生物量分别显著提高了20.66%和8.93%,柑橘地上部和总氮积累量分别提高了13.57%和6.21%;与添加铵态氮相比,添加硝态氮的柑橘地上部和总氮积累量分别提高了12.59%和5.64%,柑橘地上部和总生物量分别提高了15.68%和9.56%;不同水分和氮形态处理下,AMF均能促进柑橘幼苗生长和植株氮积累;与CK处理相比,AMF处理的地上部和总生物量分别提高了18.57%和15.06%,而RAMF处理分别提高了26.60%和25.48%;AMF处理柑橘地上部和总氮积累量分别较CK处理提高了19.49%和12.88%,RAMF处理分别提高了23.10%和20.10%.因此,不同水分和氮形态下,丛枝菌根真菌与柑橘根系共生均能促进柑橘幼苗生长,提高氮素积累能力.Citrus has fewer fine roots and is a plant with a strong dependence on arbuscular mycorrhizal fungi.In the report,focused on citrus orange seedlings,the chamber root box experiment was used to compare the effects of arbuscular mycorrhizal fungi(CK,AMF,RAMF)and citrus symbiosis on soil nitrogen content,plant nitrogen accumulation,and biomass under the different water treatments(Water filled pore space 60%and 80%)and two nitrogen fertilizer forms(NH_(4)^(+),NO_(3)^(-)).The results indicated that water,nitrogen fertilizer form,and arbuscular mycorrhizal fungi affect the total available nitrogen content in the soil,the biomass of citrus orange seedlings,and the total nitrogen accumulation in citrus significantly.Compared with the control treatment,the average aboveground and total biomass of citrus were significantly increased by 20.66%and 8.93%,respectively.Compared with the control treatment,the average nitrate nitrogen and total available nitrogen content in the soil treated with water were significantly reduced by 26.42%and 8.28%,respectively.The average aboveground and total nitrogen accumulation in citrus was significantly increased by 13.57%and 6.21%,respectively.Compared with NH_(4)^(+)treatment,the average aboveground and total nitrogen accumulation of citrus under NO_(3)^(-)treatment significantly increased by 12.59%and 5.64%,respectively,and the aboveground and total biomass of citrus significantly increased by 15.68%and 9.56%,respectively.Compared with the average aboveground and total biomass of CK treated citrus,RAMF treatment significantly increased by 26.60%and 25.48%,and AMF treatment significantly increased by 18.57%and 15.06%,respectively.Compared with the average aboveground and total nitrogen accumulation of CK treated citrus,RAMF treatment significantly increased by 23.10%and 20.10%,AMF treatment significantly increased by 19.49%and 12.88%,respectively.So,under the joint treatment of water and NO_(3)^(-),the interaction between AM fungi and citrus is more conducive to the accumulation of abovegr

关 键 词：丛枝菌根真菌 铵态氮 硝态氮 土壤水分含量 柑橘 氮积累 

分 类 号：S663[农业科学—果树学]