气生球状绿藻山西新记录  

New Record of Aerial Coccoid Green Algae in Shanxi

作　　者：侯英 刘美 陈美玲 王思欢 王清华 HOU Ying;LIU Mei;CHEN Meiling;WANG Sihuan;WANG Qinghua(School of Biological Science and Technology,Taiyuan Normal University,Jinzhong Shanxi 030619,China)

机构地区：[1]太原师范学院生物科学与技术学院,山西晋中030619

出　　处：《太原师范学院学报（自然科学版）》2024年第1期82-88,共7页Journal of Taiyuan Normal University:Natural Science Edition

基　　金：国家自然科学基金(32000167).

摘　　要：气生球状绿藻植物体微小,形态结构较为简单,生境适应性强.太原东山林场位于太原市东北面,是太原市绿化美化城市的生态屏障之一,具有典型的生境特征.为明确太原东山林场气生绿藻的种类组成及系统发育关系,本研究以从太原东山林场的岩石、树皮、苔藓等生境采集的气生绿藻作为材料,分离纯化得到10株气生绿藻.使用显微镜观察藻株形态特征,例如细胞形状、是否具有群体形态、蛋白核、色素体、细胞表面是否具有胶被等,并采用内转录间隔区2(ITS2)和18S rDNA基因序列进行系统发育学分析.通过传统形态学与分子系统发育学联用的方法对所获藻株进行准确鉴定,并确定各种类间的系统发育关系.经鉴定,10个藻株分别属于绿藻门下的4目7属9种,其中2株属于渡边球藻目Watanabeales,2株属于共球藻目Trebouxiales,4株属于环藻目Sphaeropleales,1株属于小球藻目Chlorellales.其中,单星藻属、耶氏球藻属为山西省新记录属,东方椭圆球藻、Desmodesmus multivariabilis、Coccomyxa viridis、Coccomyxa simplex、舒马赫伪鼠球藻和Neocystis brevis为山西省新记录种.本研究获取的大量纯培养藻株为太原东山林场的资源开发与利用提供了理论基础.The aerial coccoid green algae has a small cell size and a simple morphological structure.The east mountains forest farm is located in the northeast of Taiyuan,which is one of the ecological barriers for greening and beautifying the city.In order to clarify the species characteristics and phylogenetic relationship of coccoid green algae in this area,ten strains were isolated and purified from rocks,barks and mosses.Morphological characteristics of algae,such as cell shape,colony morphology,pyrenoid,chloroplast and mucilage envelope on the cell surface,were observed by microscope,and the molecular markers of internal transcribed spacer 2(ITS2)and 18S rDNA were used for phylogenetic analysis.Ten algae strains were identified as 9 different species belonging to 7 genera of 4 orders,of which 2 strains belonged to Watanabeales,2 strains belonged to Trebouxiales,4 strains belonged to Sphaeropleales,and 1 strain belonged to Chlorellales.Among them,the genera Coelastrella and Jenufa are new record genera in Shanxi province,while the Chloroidium orientalis,Desmodesmus multivari⁃alis,Coccomyxa viridis,Coccomyxa simplex,Pseudomuriella schumacherensis and Neocystis brevis are new record species in Shanxi province.The large number of pure cultured algae strains obtained in this study provide a theoretical basis for the resource development and utilization of the east mountains forest farm of Taiyuan.

关 键 词：球状绿藻 东山林场 形态学 系统发育学 

分 类 号：Q949.21[生物学—植物学]