育龄期细菌性阴道病伴盆腔炎性疾病患者阴道菌群和微生态改变及意义  

Changes and significance of vaginal microbiota and microecology in patients with bacterial vaginosis and pelvic inflammatory disease of childbearing age

作　　者：陈云[1] 朱一喜 袁洁姣 罗喜 唐征宇[3] Chen Yun;Zhu Yixi;Yuan Jiejiao;Luo Xi;Tang Zhengyu(Department of Gynecology,the First Affiliated Hospital of Hunan College of Traditional Chinese Medicine,Zhuzhou Hunan 412000,P.R.China;Department of Laboratory,the First Affiliated Hospital of Hunan College of Traditional Chinese Medicine,Zhuzhou Hunan 412000,P.R.China;Department of Obstetrics,the First Affiliated Hospital of Hunan College of Traditional Chinese Medicine,Zhuzhou Hunan 412000,P.R.China)

机构地区：[1]湖南中医药高等专科学校附属第一医院妇科,湖南株洲412000 [2]湖南中医药高等专科学校附属第一医院检验科,湖南株洲412000 [3]湖南中医药高等专科学校附属第一医院产科,湖南株洲412000

出　　处：《中国计划生育和妇产科》2024年第5期79-82,共4页Chinese Journal of Family Planning & Gynecotokology

摘　　要：目的分析育龄期细菌性阴道病(BV)伴盆腔炎性疾病(PID)患者阴道菌群和微生态改变情况及意义。方法选取2020年9月至2022年12月湖南中医药高等专科学校附属第一医院76例育龄期BV患者,按照是否伴PID,分为BV组(33例)与BV伴PID组(43例),并选取同期50例育龄期体检健康者作为对照组,比较3组阴道菌群密集度、阴道菌群多样性及16S rRNA测序结果。结果BV伴PID组、BV组与对照组阴道菌群密集度分级、阴道菌群多样性分级比较差异有统计学意义(P<0.05)。与对照组相比,BV组、BV伴PID组厚壁菌门减少,并且放线菌门增多;对照组无加德纳菌,BV组、BV伴PID组加德纳菌较多,且乳酸菌相较于对照组减少,BV组厌氧菌(如加特纳菌、普雷沃氏菌等)增多,BV伴PID组厌氧菌(如G.vaginallis等)存在增加趋势,卷曲乳杆菌存在减少趋势。结论育龄期BV及BV伴PID患者阴道菌群密集度、阴道菌群多样性增加,微生态平衡发生变化,厌氧菌增多,其检测可为疾病诊治提供指导。Objective To analyze the changes and significance of vaginal microbiota and microecology in patients with bacterial vaginosis(BV)and pelvic inflammatory disease(PID)of childbearing age.Methods A total of 76 patients with BV of childbearing age who were admitted to the First Affiliated Hospital of Hunan College of Traditional Chinese Medicine from September 2020 to December 2022 were selected.They were divided into BV group(33 cases)and BV with PID group(43 cases)according to the presence or absence of PID.At the same time,50 healthy individuals of childbearing age were selected as the control group.The density and diversity of vaginal microbiota,and 16S rRNA sequencing results of the three groups were compared.Results There were significant differences in the classification of the density and diversity of vaginal microbiota among the BV with PID group,the BV group and the control group(P<0.05).Compared with the control group,Firmicutes was less and Actinobacteria was more in the BV group and the BV with PID group.No Gardnerella was found in the control group,while there was much Gardnerella in the BV group and the BV with PID group.Lactobacillus was less in the BV group and the BV with PID group than in the control group.Anaerobic bacteria such as G.vaginalis and P.vaginalis in the BV group increased.In the BV with PID group,anaerobic bacteria like G.vaginalis showed an increasing trend,and L.crispatus showed a decreasing trend.Conclusion The density and diversity of vaginal microbiota in patients with BV and patients with BV complicated with PID of childbearing age are increased.Their microecological balance is changed,and anaerobic bacteria are increased.The detection of vaginal microbiota and microecology can provide guidance for diagnosis and treatment.

关 键 词：育龄期 细菌性阴道病 盆腔炎性疾病 阴道菌群 微生态 

分 类 号：R711.33[医药卫生—妇产科学]